![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 2, 678-687, February 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
*Departamento de Inmunología, Hospital Universitario de la Princesa, 28006 Madrid, Spain
Submitted July 24, 2003;
Revised November 7, 2003;
Accepted November 7, 2003
Monitoring Editor: Martin Schwartz
The extracellular matrix (ECM) distinctly modulates membrane type 1-matrix metalloproteinase (MT1-MMP) in human endothelial cells (ECs). Herein, ECM-dependent RhoA activation is shown to regulate MT1-MMP localization and activity as well as clathrin-independent internalization in confluent ECs. In this regard, caveolae are revealed as the major MT1-MMP endocytic pathway in human ECs. Thus, MT1-MMP is present at caveolae with caveolin-1 and both proteins together with 
v
3 integrin colocalize at endothelial motility-associated extensions. Remarkably, caveolae traffic is required for proper MT1-MMP localization, activity, and function in migratory ECs as demonstrated by both treatment with caveolae-disrupting agents or selective targeting caveolin-1 expression by interference RNA. Thus, caveolae-mediated traffic constitutes a novel mechanism for MT1-MMP regulation in ECs during angiogenesis.
Abbreviations used: cdx, cyclodextrin; COL I, collagen type I; EC, endothelial cell; ECM, extracellular matrix; GEL, gelatin; MMP, matrix metalloproteinase; MT1-MMP, membrane-type 1 matrix metalloproteinase; TnfR, transferrin receptor.
Present address: Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
* Corresponding author. E-mail address: agarciaa.hlpr{at}salud.madrid.org.
This article has been cited by other articles:
|
|
 |
|
  H. Yamaguchi, Y. Takeo, S. Yoshida, Z. Kouchi, Y. Nakamura, and K. Fukami Lipid Rafts and Caveolin-1 Are Required for Invadopodia Formation and Extracellular Matrix Degradation by Human Breast Cancer Cells Cancer Res., November 15, 2009; 69(22): 8594 - 8602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Deshmukh, A. McLachlan, J. J. Atkinson, W. D. Hardie, T. R. Korfhagen, M. Dietsch, Y. Liu, P. Y. P. Di, S. C. Wesselkamper, M. T. Borchers, et al. Matrix Metalloproteinase-14 Mediates a Phenotypic Shift in the Airways to Increase Mucin Production Am. J. Respir. Crit. Care Med., November 1, 2009; 180(9): 834 - 845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Alcazar, A. M. Hawkridge, T. S. Collier, S. W. Cousins, S. K. Bhattacharya, D. C. Muddiman, and M. E. Marin-Castano Proteomics Characterization of Cell Membrane Blebs in Human Retinal Pigment Epithelium Cells Mol. Cell. Proteomics, October 1, 2009; 8(10): 2201 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Lizarbe, C. Tarin, M. Gomez, B. Lavin, E. Aracil, L. M. Orte, and C. Zaragoza Nitric Oxide Induces the Progression of Abdominal Aortic Aneurysms through the Matrix Metalloproteinase Inducer EMMPRIN Am. J. Pathol., October 1, 2009; 175(4): 1421 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Poincloux, F. Lizarraga, and P. Chavrier Matrix invasion by tumour cells: a focus on MT1-MMP trafficking to invadopodia J. Cell Sci., September 1, 2009; 122(17): 3015 - 3024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yanez-Mo, O. Barreiro, P. Gonzalo, A. Batista, D. Megias, L. Genis, N. Sachs, M. Sala-Valdes, M. A. Alonso, M. C. Montoya, et al. MT1-MMP collagenolytic activity is regulated through association with tetraspanin CD151 in primary endothelial cells Blood, October 15, 2008; 112(8): 3217 - 3226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Lizarbe, C. Garcia-Rama, C. Tarin, M. Saura, E. Calvo, J. A. Lopez, C. Lopez-Otin, A. R. Folgueras, S. Lamas, and C. Zaragoza Nitric oxide elicits functional MMP-13 protein-tyrosine nitration during wound repair FASEB J, September 1, 2008; 22(9): 3207 - 3215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ispanovic, D. Serio, and T. L. Haas Cdc42 and RhoA have opposing roles in regulating membrane type 1-matrix metalloproteinase localization and matrix metalloproteinase-2 activation Am J Physiol Cell Physiol, September 1, 2008; 295(3): C600 - C610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alfranca, J. M. Lopez-Oliva, L. Genis, D. Lopez-Maderuelo, I. Mirones, D. Salvado, A. J. Quesada, A. G. Arroyo, and J. M. Redondo PGE2 induces angiogenesis via MT1-MMP-mediated activation of the TGF{beta}/Alk5 signaling pathway Blood, August 15, 2008; 112(4): 1120 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. W.M. van Hinsbergh and P. Koolwijk Endothelial sprouting and angiogenesis: matrix metalloproteinases in the lead Cardiovasc Res, May 1, 2008; 78(2): 203 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takino, H. Saeki, H. Miyamori, T. Kudo, and H. Sato Inhibition of Membrane-Type 1 Matrix Metalloproteinase at Cell-Matrix Adhesions Cancer Res., December 15, 2007; 67(24): 11621 - 11629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Genis, P. Gonzalo, A. S. Tutor, B. G. Galvez, A. Martinez-Ruiz, C. Zaragoza, S. Lamas, K. Tryggvason, S. S. Apte, and A. G. Arroyo Functional interplay between endothelial nitric oxide synthase and membrane type 1 matrix metalloproteinase in migrating endothelial cells Blood, October 15, 2007; 110(8): 2916 - 2923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Nyalendo, M. Michaud, E. Beaulieu, C. Roghi, G. Murphy, D. Gingras, and R. Beliveau Src-dependent Phosphorylation of Membrane Type I Matrix Metalloproteinase on Cytoplasmic Tyrosine 573: ROLE IN ENDOTHELIAL AND TUMOR CELL MIGRATION J. Biol. Chem., May 25, 2007; 282(21): 15690 - 15699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grande-Garcia, A. Echarri, J. de Rooij, N. B. Alderson, C. M. Waterman-Storer, J. M. Valdivielso, and M. A. del Pozo Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases J. Cell Biol., May 21, 2007; 177(4): 683 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miki, Y. Takegami, K. Okawa, T. Muraguchi, M. Noda, and C. Takahashi The Reversion-inducing Cysteine-rich Protein with Kazal Motifs (RECK) Interacts with Membrane Type 1 Matrix Metalloproteinase and CD13/Aminopeptidase N and Modulates Their Endocytic Pathways J. Biol. Chem., April 20, 2007; 282(16): 12341 - 12352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nie, J. Pei, M. Blumenthal, and D. Pei Complete Restoration of Cell Surface Activity of Transmembrane-truncated MT1-MMP by a Glycosylphosphatidylinositol Anchor: IMPLICATIONS FOR MT1-MMP-MEDIATED PROMMP2 ACTIVATION AND COLLAGENOLYSIS IN THREE-DIMENSIONS J. Biol. Chem., March 2, 2007; 282(9): 6438 - 6443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ning, T. Buranda, and L. G. Hudson Activated Epidermal Growth Factor Receptor Induces Integrin {alpha}2 Internalization via Caveolae/Raft-dependent Endocytic Pathway J. Biol. Chem., March 2, 2007; 282(9): 6380 - 6387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ispanovic and T. L. Haas JNK and PI3K differentially regulate MMP-2 and MT1-MMP mRNA and protein in response to actin cytoskeleton reorganization in endothelial cells Am J Physiol Cell Physiol, October 1, 2006; 291(4): C579 - C588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. W.M. van Hinsbergh, M. A. Engelse, and P. H.A. Quax Pericellular Proteases in Angiogenesis and Vasculogenesis Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 716 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lafleur, F. A. Mercuri, N. Ruangpanit, M. Seiki, H. Sato, and E. W. Thompson Type I Collagen Abrogates the Clathrin-mediated Internalization of Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) via the MT1-MMP Hemopexin Domain J. Biol. Chem., March 10, 2006; 281(10): 6826 - 6840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Osenkowski, S. O. Meroueh, D. Pavel, S. Mobashery, and R. Fridman Mutational and Structural Analyses of the Hinge Region of Membrane Type 1-Matrix Metalloproteinase and Enzyme Processing J. Biol. Chem., July 15, 2005; 280(28): 26160 - 26168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cambier, S. Gline, D. Mu, R. Collins, J. Araya, G. Dolganov, S. Einheber, N. Boudreau, and S. L. Nishimura Integrin {alpha}v{beta}8-Mediated Activation of Transforming Growth Factor-{beta} by Perivascular Astrocytes: An Angiogenic Control Switch Am. J. Pathol., June 1, 2005; 166(6): 1883 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lehti, E. Allen, H. Birkedal-Hansen, K. Holmbeck, Y. Miyake, T.-H. Chun, and S. J. Weiss An MT1-MMP-PDGF receptor-{beta} axis regulates mural cell investment of the microvasculature Genes & Dev., April 15, 2005; 19(8): 979 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cavallo-Medved, J. Mai, J. Dosescu, M. Sameni, and B. F. Sloane Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells J. Cell Sci., April 1, 2005; 118(7): 1493 - 1503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Williams and M. P. Lisanti Caveolin-1 in oncogenic transformation, cancer, and metastasis Am J Physiol Cell Physiol, March 1, 2005; 288(3): C494 - C506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. G. Galvez, L. Genis, S. Matias-Roman, S. A. Oblander, K. Tryggvason, S. S. Apte, and A. G. Arroyo Membrane Type 1-Matrix Metalloproteinase Is Regulated by Chemokines Monocyte-Chemoattractant Protein-1/CCL2 and Interleukin-8/CXCL8 in Endothelial Cells during Angiogenesis J. Biol. Chem., January 14, 2005; 280(2): 1292 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Emonard, G. Bellon, L. Troeberg, A. Berton, A. Robinet, P. Henriet, E. Marbaix, K. Kirkegaard, L. Patthy, Y. Eeckhout, et al. Low Density Lipoprotein Receptor-related Protein Mediates Endocytic Clearance of Pro-MMP-2{middle dot}TIMP-2 Complex through a Thrombospondin-independent Mechanism J. Biol. Chem., December 24, 2004; 279(52): 54944 - 54951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Labrecque, C. Nyalendo, S. Langlois, Y. Durocher, C. Roghi, G. Murphy, D. Gingras, and R. Beliveau Src-mediated Tyrosine Phosphorylation of Caveolin-1 Induces Its Association with Membrane Type 1 Matrix Metalloproteinase J. Biol. Chem., December 10, 2004; 279(50): 52132 - 52140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Williams, F. Medina, I. Badano, R. B. Hazan, J. Hutchinson, W. J. Muller, N. G. Chopra, P. E. Scherer, R. G. Pestell, and M. P. Lisanti Caveolin-1 Gene Disruption Promotes Mammary Tumorigenesis and Dramatically Enhances Lung Metastasis in Vivo: ROLE OF CAV-1 IN CELL INVASIVENESS AND MATRIX METALLOPROTEINASE (MMP-2/9) SECRETION J. Biol. Chem., December 3, 2004; 279(49): 51630 - 51646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gonzalez, A. Nagiel, A. J. Lin, D. E. Golan, and T. Michel Small Interfering RNA-mediated Down-regulation of Caveolin-1 Differentially Modulates Signaling Pathways in Endothelial Cells J. Biol. Chem., September 24, 2004; 279(39): 40659 - 40669. [Abstract] [Full Text] [PDF]
|
|
