![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 2, 721-733, February 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021
Submitted July 16, 2003;
Revised September 26, 2003;
Accepted September 27, 2003
Monitoring Editor: Suzanne Pfeffer
Although the distribution of the cation-independent mannose 6-phosphate receptor (CI-MPR) has been well studied, its intracellular itinerary and trafficking kinetics remain uncertain. In this report, we describe the endocytic trafficking and steady-state localization of a chimeric form of the CI-MPR containing the ecto-domain of the bovine CI-MPR and the murine transmembrane and cytoplasmic domains expressed in a CHO cell line. Detailed confocal microscopy analysis revealed that internalized chimeric CI-MPR overlaps almost completely with the endogenous CI-MPR but only partially with individual markers for the trans-Golgi network or other endosomal compartments. After endocytosis, the chimeric receptor first enters sorting endosomes, and it then accumulates in the endocytic recycling compartment. A large fraction of the receptors return to the plasma membrane, but some are delivered to the trans-Golgi network and/or late endosomes. Over the course of an hour, the endocytosed receptors achieve their steady-state distribution. Importantly, the receptor does not start to colocalize with late endosomal markers until after it has passed through the endocytic recycling compartment. In CHO cells, only a small fraction of the receptor is ever detected in endosomes bearing substrates destined for lysosomes (kinetically defined late endosomes). These data demonstrate that CI-MPR takes a complex route that involves multiple sorting steps in both early and late endosomes.
* These authors contributed equally to this work.
Present address: Department of Molecular Oncology, Genentech, Inc., 1 DNA Way, MS 42, South San Francisco, CA 94080.
Corresponding author. E-mail address: frmaxfie{at}med.cornell.edu.
This article has been cited by other articles:
|
|
 |
|
  L. Klotz, S. Hucke, D. Thimm, S. Classen, A. Gaarz, J. Schultze, F. Edenhofer, C. Kurts, T. Klockgether, A. Limmer, et al. Increased Antigen Cross-Presentation but Impaired Cross-Priming after Activation of Peroxisome Proliferator-Activated Receptor {gamma} Is Mediated by Up-Regulation of B7H1 J. Immunol., July 1, 2009; 183(1): 129 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. C. Ikonomov, J. Fligger, D. Sbrissa, R. Dondapati, K. Mlak, R. Deeb, and A. Shisheva Kinesin Adapter JLP Links PIKfyve to Microtubule-based Endosome-to-Trans-Golgi Network Traffic of Furin J. Biol. Chem., February 6, 2009; 284(6): 3750 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-d. Zhu, G. Salazar, S. A. Zlatic, B. Fiza, M. M. Doucette, C. J. Heilman, A. I. Levey, V. Faundez, and S. W. L'Hernault SPE-39 Family Proteins Interact with the HOPS Complex and Function in Lysosomal Delivery Mol. Biol. Cell, February 1, 2009; 20(4): 1223 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Bujny, P. A. Ewels, S. Humphrey, N. Attar, M. A. Jepson, and P. J. Cullen Sorting nexin-1 defines an early phase of Salmonella-containing vacuole-remodeling during Salmonella infection J. Cell Sci., June 15, 2008; 121(12): 2027 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. G. Ganley, E. Espinosa, and S. R. Pfeffer A syntaxin 10 SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells J. Cell Biol., January 10, 2008; 180(1): 159 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hambleton, S. P. Steinberg, M. D. Gershon, and A. A. Gershon Cholesterol Dependence of Varicella-Zoster Virion Entry into Target Cells J. Virol., July 15, 2007; 81(14): 7548 - 7558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. T. Tran, Q. Zeng, and W. Hong VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes J. Cell Sci., March 15, 2007; 120(6): 1028 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Rutherford, C. Traer, T. Wassmer, K. Pattni, M. V. Bujny, J. G. Carlton, H. Stenmark, and P. J. Cullen The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport J. Cell Sci., October 1, 2006; 119(19): 3944 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Y. Chen, A. Ieraci, M. Tanowitz, and F. S. Lee A Novel Endocytic Recycling Signal Distinguishes Biological Responses of Trk Neurotrophin Receptors Mol. Biol. Cell, December 1, 2005; 16(12): 5761 - 5772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Carlton, M. V. Bujny, B. J. Peter, V. M. J. Oorschot, A. Rutherford, R. S. Arkell, J. Klumperman, H. T. McMahon, and P. J. Cullen Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport J. Cell Sci., October 1, 2005; 118(19): 4527 - 4539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Liu, R. Cescato, D. A. Dewi, J. Rivier, J.-C. Reubi, and A. Schonbrunn Receptor Signaling and Endocytosis Are Differentially Regulated by Somatostatin Analogs Mol. Pharmacol., July 1, 2005; 68(1): 90 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Raja, S. S. Metkar, S. Honing, B. Wang, W. A. Russin, N. H. Pipalia, C. Menaa, M. Belting, X. Cao, R. Dressel, et al. A Novel Mechanism for Protein Delivery: GRANZYME B UNDERGOES ELECTROSTATIC EXCHANGE FROM SERGLYCIN TO TARGET CELLS J. Biol. Chem., May 27, 2005; 280(21): 20752 - 20761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kzhyshkowska, A. Gratchev, J.-H. Martens, O. Pervushina, S. Mamidi, S. Johansson, K. Schledzewski, B. Hansen, X. He, J. Tang, et al. Stabilin-1 localizes to endosomes and the trans-Golgi network in human macrophages and interacts with GGA adaptors J. Leukoc. Biol., December 1, 2004; 76(6): 1151 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Austin, A. M. De Maziere, P. I. Pisacane, S. M. van Dijk, C. Eigenbrot, M. X. Sliwkowski, J. Klumperman, and R. H. Scheller Endocytosis and Sorting of ErbB2 and the Site of Action of Cancer Therapeutics Trastuzumab and Geldanamycin Mol. Biol. Cell, December 1, 2004; 15(12): 5268 - 5282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Natarajan and A. D. Linstedt A Cycling cis-Golgi Protein Mediates Endosome-to-Golgi Traffic Mol. Biol. Cell, November 1, 2004; 15(11): 4798 - 4806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lu, G. Tai, and W. Hong Autoantigen Golgin-97, an Effector of Arl1 GTPase, Participates in Traffic from the Endosome to the Trans-Golgi Network Mol. Biol. Cell, October 1, 2004; 15(10): 4426 - 4443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Moore, E. E. Millman, E. Alpizar-Foster, W. Dai, and B. J. Knoll Rab11 regulates the recycling and lysosome targeting of {beta}2-adrenergic receptors J. Cell Sci., July 1, 2004; 117(15): 3107 - 3117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N.J. Seaman Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer J. Cell Biol., April 12, 2004; 165(1): 111 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Arighi, L. M. Hartnell, R. C. Aguilar, C. R. Haft, and J. S. Bonifacino Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor J. Cell Biol., April 12, 2004; 165(1): 123 - 133. [Abstract] [Full Text] [PDF]
|
|
