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Vol. 15, Issue 2, 851-860, February 2004
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* Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom;
The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom; and
MRC Radiation and Genome Stability Unit, Harwell, Didcot, Oxon OX11 ORD, United Kingdom
Submitted August 8, 2003;
Revised September 15, 2003;
Accepted October 6, 2003
Monitoring Editor: Pamela Silver
A coordinated transcriptional response to DNA-damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) by using DNA microarrays. We identified 
200 genes whose transcript levels were significantly altered at least twofold in response to 500 Gy of gamma IR in a temporally defined manner. The majority of induced genes were core environmental stress response genes, whereas the remaining genes define a transcriptional response to DNA damage in fission yeast. Surprisingly, few DNA repair and checkpoint genes were transcriptionally modulated in response to IR. We define a role for the stress-activated mitogen-activated protein kinase Sty1/Spc1 and the DNA damage checkpoint kinase Rad3 in regulating core environmental stress response genes and IR-specific response genes, both independently and in concert. These findings suggest a complex network of regulatory pathways coordinate gene expression responses to IR in eukaryotes.
Online version of this article contains supplemental tables. Online version is available at www.molbiolcell.org.
Corresponding author. E-mail address: t.humphrey{at}har.mrc.ac.uk.
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