![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 3, 1324-1333, March 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Laboratoire de Neurogenèse et Morphogenèse au cours du Développement et chez l'Adulte, Unité Mixte Recherche 6156 Centre National de la Recherche Scientifique, Institut de Biologie du Développment de Marseille, Université de la Méditerranée, Campus de Luminy, 13288 Marseille, France
Submitted April 16, 2003;
Revised September 26, 2003;
Accepted October 24, 2003
Monitoring Editor: Mark Ginsberg
Crumbs is an apical transmembrane protein crucial for epithelial morphogenesis in Drosophila melanogaster embryos. A protein with all the characteristics for a Crumbs homologue has been identified from patients suffering from retinitis pigmentosa group 12, but this protein (CRB1) is only expressed in retina and some parts of the brain, both in human and mouse. Here, we describe CRB3, another Crumbs homologue that is preferentially expressed in epithelial tissues and skeletal muscles in human. CRB3 shares the conserved cytoplasmic domain with other Crumbs but exhibits a very short extracellular domain without the EGF- and laminin A-like G repeats present in the other Crumbs. CRB3 is localized to the apical and subapical area of epithelial cells from the mouse and human intestine, suggesting that it could play a role in epithelial morphogenesis. Indeed, expression of CRB3 or of a chimera containing the extracellular domain of the neurotrophin receptor p75NTR and the transmembrane and cytoplasmic domains of CRB3 led to a slower development of functional tight junctions in Madin-Darby canine kidney cells. This phenotype relied on the presence of CRB3 four last amino acids (ERLI) that are involved in a direct interaction with Par6, a regulator of epithelial polarity and tight junction formation. Thus, CRB3, through its cytoplasmic domain and its interactors, plays a role in apical membrane morphogenesis and tight junction regulation.
Abbreviations used: MDCK, Madin-Darby kidney cell; NTR, neurotrophin receptor; PATJ, protein associated to tight junctions; PDZ, PSD95/Discs large/ZO-1; PRC, photoreceptor cell; TJ, tight junction.
* These authors contributed equally to this work.
Corresponding author. E-mail address: lebivic{at}ibdm.univ-mrs.fr.
This article has been cited by other articles:
|
|
 |
|
  N. Bit-Avragim, N. Hellwig, F. Rudolph, C. Munson, D. Y. S. Stainier, and S. Abdelilah-Seyfried Divergent polarization mechanisms during vertebrate epithelial development mediated by the Crumbs complex protein Nagie oko J. Cell Sci., August 1, 2008; 121(15): 2503 - 2510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Bulgakova, O. Kempkens, and E. Knust Multiple domains of Stardust differentially mediate localisation of the Crumbs-Stardust complex during photoreceptor development in Drosophila J. Cell Sci., June 15, 2008; 121(12): 2018 - 2026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Karp, T. T. Tan, R. Mathew, D. Nelson, C. Mukherjee, K. Degenhardt, V. Karantza-Wadsworth, and E. White Role of the Polarity Determinant Crumbs in Suppressing Mammalian Epithelial Tumor Progression Cancer Res., June 1, 2008; 68(11): 4105 - 4115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sfakianos, A. Togawa, S. Maday, M. Hull, M. Pypaert, L. Cantley, D. Toomre, and I. Mellman Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells J. Cell Biol., December 17, 2007; 179(6): 1133 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fan, V. Fogg, Q. Wang, X.-W. Chen, C.-J. Liu, and B. Margolis A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin {beta} interactions J. Cell Biol., July 24, 2007; 178(3): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Cline and W. J. Nelson Characterization of Mammalian Par 6 as a Dual-Location Protein Mol. Cell. Biol., June 15, 2007; 27(12): 4431 - 4443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Lee, N. F. Silva-Gagliardi, U. Tepass, C. J. McGlade, and K. V. Anderson The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo Development, June 1, 2007; 134(11): 2007 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matter and M. S. Balda Epithelial tight junctions, gene expression and nucleo-junctional interplay J. Cell Sci., May 1, 2007; 120(9): 1505 - 1511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Massey-Harroche, M.-H. Delgrossi, L. Lane-Guermonprez, J.-P. Arsanto, J.-P. Borg, M. Billaud, and A. Le Bivic Evidence for a molecular link between the tuberous sclerosis complex and the Crumbs complex Hum. Mol. Genet., March 1, 2007; 16(5): 529 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, X.-W. Chen, and B. Margolis PALS1 Regulates E-Cadherin Trafficking in Mammalian Epithelial Cells Mol. Biol. Cell, March 1, 2007; 18(3): 874 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Hsu, J. J. Willoughby, A. K. Christensen, and A. M. Jensen Mosaic Eyes is a novel component of the Crumbs complex and negatively regulates photoreceptor apical size Development, December 15, 2006; 133(24): 4849 - 4859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Straight, J. N. Pieczynski, E. L. Whiteman, C.-J. Liu, and B. Margolis Mammalian Lin-7 Stabilizes Polarity Protein Complexes J. Biol. Chem., December 8, 2006; 281(49): 37738 - 37747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Richard, R. Roepman, W. M. Aartsen, A. G.S.H. van Rossum, A. I. den Hollander, E. Knust, J. Wijnholds, and F. P.M. Cremers Towards understanding CRUMBS function in retinal dystrophies Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R235 - R243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Afonso and D. Henrique PAR3 acts as a molecular organizer to define the apical domain of chick neuroepithelial cells J. Cell Sci., October 15, 2006; 119(20): 4293 - 4304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Beers and K. Kemphues Depletion of the co-chaperone CDC-37 reveals two modes of PAR-6 cortical association in C. elegans embryos Development, October 1, 2006; 133(19): 3745 - 3754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G.S.H. van Rossum, W. M. Aartsen, J. Meuleman, J. Klooster, A. Malysheva, I. Versteeg, J.-P. Arsanto, A. Le Bivic, and J. Wijnholds Pals1/Mpp5 is required for correct localization of Crb1 at the subapical region in polarized Muller glia cells Hum. Mol. Genet., September 15, 2006; 15(18): 2659 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamanaka, Y. Horikoshi, N. Izumi, A. Suzuki, K. Mizuno, and S. Ohno Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity J. Cell Sci., May 15, 2006; 119(10): 2107 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hirose, M. Karasawa, Y. Sugitani, M. Fujisawa, K. Akimoto, S. Ohno, and T. Noda PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains Development, April 1, 2006; 133(7): 1389 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suzuki and S. Ohno The PAR-aPKC system: lessons in polarity. J. Cell Sci., March 15, 2006; 119(Pt 6): 979 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Michel, J.-P. Arsanto, D. Massey-Harroche, C. Beclin, J. Wijnholds, and A. Le Bivic PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells J. Cell Sci., September 1, 2005; 118(17): 4049 - 4057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ozdamar, R. Bose, M. Barrios-Rodiles, H.-R. Wang, Y. Zhang, and J. L. Wrana Regulation of the Polarity Protein Par6 by TGF{beta} Receptors Controls Epithelial Cell Plasticity Science, March 11, 2005; 307(5715): 1603 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Vogelmann and W. J. Nelson Fractionation of the Epithelial Apical Junctional Complex: Reassessment of Protein Distributions in Different Substructures Mol. Biol. Cell, February 1, 2005; 16(2): 701 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gao and I. G. Macara Isoforms of the Polarity Protein Par6 Have Distinct Functions J. Biol. Chem., October 1, 2004; 279(40): 41557 - 41562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sotillos, M. T. Diaz-Meco, E. Caminero, J. Moscat, and S. Campuzano DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila J. Cell Biol., August 16, 2004; 166(4): 549 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. van de Pavert, A. Kantardzhieva, A. Malysheva, J. Meuleman, I. Versteeg, C. Levelt, J. Klooster, S. Geiger, M. W. Seeliger, P. Rashbass, et al. Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure J. Cell Sci., August 15, 2004; 117(18): 4169 - 4177. [Abstract] [Full Text] [PDF]
|
|
