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Vol. 15, Issue 3, 1445-1458, March 2004

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Reconstituted TOM Core Complex and Tim9/Tim10 Complex of Mitochondria Are Sufficient for Translocation of the ADP/ATP Carrier across Membranes

Andreja Vasiljev ^*, Uwe Ahting ^*, Frank E. Nargang , Nancy E. Go , Shukry J. Habib ^*, Christian Kozany ^*, Valérie Panneels , Irmgard Sinning , Holger Prokisch ^* , Walter Neupert ^*, Stephan Nussberger ^* ||, and Doron Rapaport ^* ¶

^* Institut für Physiologische Chemie der Universität München, D-81377 Munich, Germany; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9; and Biochemie-Zentrum Heidelberg, INF328, 69120 Heidelberg, Germany

Submitted May 2, 2003; Revised October 23, 2003; Accepted October 24, 2003
Monitoring Editor: Thomas Fox

Precursor proteins of the solute carrier family and of channel forming Tim components are imported into mitochondria in two main steps. First, they are translocated through the TOM complex in the outer membrane, a process assisted by the Tim9/Tim10 complex. They are passed on to the TIM22 complex, which facilitates their insertion into the inner membrane. In the present study, we have analyzed the function of the Tim9/Tim10 complex in the translocation of substrates across the outer membrane of mitochondria. The purified TOM core complex was reconstituted into lipid vesicles in which purified Tim9/Tim10 complex was entrapped. The precursor of the ADP/ATP carrier (AAC) was found to be translocated across the membrane of such lipid vesicles. Thus, these components are sufficient for translocation of AAC precursor across the outer membrane. Peptide libraries covering various substrate proteins were used to identify segments that are bound by Tim9/Tim10 complex upon translocation through the TOM complex. The patterns of binding sites on the substrate proteins suggest a mechanism by which portions of membrane-spanning segments together with flanking hydrophilic segments are recognized and bound by the Tim9/Tim10 complex as they emerge from the TOM complex into the intermembrane space.

Present address: Institute of Human Genetics, Technical University of Munich, D-81675, Munich, Germany; ^||Biologisches Institut Abt. Biophysik, Universität Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, Germany.

^|| Present address: Biologisches Institut Abt. Biophysik, Universität Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, Germany.

^¶ Corresponding author. E-mail address: rapaport{at}bio.med.unimuenchen.de.

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