![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 4, 1969-1980, April 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Stra
e, D-35043 Marburg, Germany
Submitted September 17, 2003;
Revised December 22, 2003;
Accepted December 27, 2003
Monitoring Editor: David Drubin
The microtubule cytoskeleton supports cellular morphogenesis and polar growth, but the underlying mechanisms are not understood. In a screen for morphology mutants defective in microtubule organization in the fungus Ustilago maydis, we identified eca1 that encodes a sarcoplasmic/endoplasmic calcium ATPase. Eca1 resides in the endoplasmic reticulum and restores growth of a yeast mutant defective in calcium homeostasis. Deletion of eca1 resulted in elevated cytosolic calcium levels and a severe growth and morphology defect. While F-actin and myosin V distribution is unaffected, 
eca1 mutants contain longer and disorganized microtubules that show increased rescue and reduced catastrophe frequencies. Morphology can be restored by inhibition of Ca2+/calmodulin-dependent kinases or destabilizing microtubules, indicating that calcium-dependent alterations in dynamic instability are a major cause of the growth defect. Interestingly, dynein mutants show virtually identical changes in microtubule dynamics and dynein-dependent ER motility was drastically decreased in eca1. This indicates a connection between calcium signaling, dynein, and microtubule organization in morphogenesis of U. maydis.
Abbreviations used: CaM, calmodulin; CaMK, Ca2+-calmodulin-dependent kinase; ER, endoplasmic reticulum; CFP, cyan fluorescent protein; GFP, green fluorescent protein; MT, microtubule; SERCA, sarcoplasmic/endoplasmic reticulum Ca2+ ATPase; ts, temperature sensitive; YFP, yellow fluorescent protein.
* Present address: Hanulova 1, 84101 Bratislava, Slovakia
Present address: Wellcome Trust Centre for Cell Biology, ICMB, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, Scotland, UK
Present address: Institut für Zellbiologie, LMU, Schillerstr. 42, D-80336 München, Germany.
Corresponding author. E-mail address: gero.steinberg{at}staff.unimarburg.de.
This article has been cited by other articles:
|
|
 |
|
  W. Fan, A. Idnurm, J. Breger, E. Mylonakis, and J. Heitman Eca1, a Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase, Is Involved in Stress Tolerance and Virulence in Cryptococcus neoformans Infect. Immun., July 1, 2007; 75(7): 3394 - 3405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Fink and G. Steinberg Dynein-dependent Motility of Microtubules and Nucleation Sites Supports Polarization of the Tubulin Array in the Fungus Ustilago maydis Mol. Biol. Cell, July 1, 2006; 17(7): 3242 - 3253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Straube, G. Hause, G. Fink, and G. Steinberg Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo Mol. Biol. Cell, February 1, 2006; 17(2): 907 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Kraus, C. B. Nichols, and J. Heitman Calcium- and Calcineurin-Independent Roles for Calmodulin in Cryptococcus neoformans Morphogenesis and High-Temperature Growth Eukaryot. Cell, June 1, 2005; 4(6): 1079 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Fuchs, I. Manns, and G. Steinberg Microtubules Are Dispensable for the Initial Pathogenic Development but Required for Long-Distance Hyphal Growth in the Corn Smut Fungus Ustilago maydis Mol. Biol. Cell, June 1, 2005; 16(6): 2746 - 2758. [Abstract] [Full Text] [PDF]
|
|
