![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, Issue 5, 2423-2435, May 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544
Submitted September 25, 2003;
Revised February 9, 2004;
Accepted February 23, 2004
Monitoring Editor: Vivek Malhotra
The conserved oligomeric Golgi (COG) complex is a soluble hetero-octamer associated with the cytoplasmic surface of the Golgi. Mammalian somatic cell mutants lacking the Cog1 (ldlB) or Cog2 (ldlC) subunits exhibit pleiotropic defects in Golgi-associated glycoprotein and glycolipid processing that suggest COG is involved in the localization, transport, and/or function of multiple Golgi processing proteins. We have identified a set of COG-sensitive, integral membrane Golgi proteins called GEARs (mannosidase II, GOS-28, GS15, GPP130, CASP, giantin, and golgin-84) whose abundances were reduced in the mutant cells and, in some cases, increased in COG-overexpressing cells. In the mutants, some GEARs were abnormally localized in the endoplasmic reticulum and were degraded by proteasomes. The distributions of the GEARs were altered by small interfering RNA depletion of 
-COP in wild-type cells under conditions in which COG-insensitive proteins were unaffected. Furthermore, synthetic phenotypes arose in mutants deficient in both -COP and either Cog1 or Cog2. COG and COPI may work in concert to ensure the proper retention or retrieval of a subset of proteins in the Golgi, and COG helps prevent the endoplasmic reticulum accumulation and degradation of some GEARs.
Online version of this article contains supporting material. Online version is available at www.molbiolcell.org.
Corresponding author. E-mail address: krieger{at}mit.edu.
This article has been cited by other articles:
|
|
 |
|
  S. Tamai, H. Iida, S. Yokota, T. Sayano, S. Kiguchiya, N. Ishihara, J.-I. Hayashi, K. Mihara, and T. Oka Characterization of the mitochondrial protein LETM1, which maintains the mitochondrial tubular shapes and interacts with the AAA-ATPase BCS1L J. Cell Sci., August 1, 2008; 121(15): 2588 - 2600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ishikawa, C. Machida, Y. Yoshioka, T. Ueda, A. Nakano, and Y. Machida EMBRYO YELLOW gene, encoding a subunit of the conserved oligomeric Golgi complex, is required for appropriate cell expansion and meristem organization in Arabidopsis thaliana. Genes Cells, June 1, 2008; 13(6): 521 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oka, T. Sayano, S. Tamai, S. Yokota, H. Kato, G. Fujii, and K. Mihara Identification of a Novel Protein MICS1 that is Involved in Maintenance of Mitochondrial Morphology and Apoptotic Release of Cytochrome c Mol. Biol. Cell, June 1, 2008; 19(6): 2597 - 2608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Peng, C. Togawa, K. Zhang, and S. K. Kurdistani Regulators of Cellular Levels of Histone Acetylation in Saccharomyces cerevisiae Genetics, May 1, 2008; 179(1): 277 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shestakova, E. Suvorova, O. Pavliv, G. Khaidakova, and V. Lupashin Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability J. Cell Biol., December 17, 2007; 179(6): 1179 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Cavanaugh, X. Chen, B. C. Richardson, D. Ungar, I. Pelczer, J. Rizo, and F. M. Hughson Structural Analysis of Conserved Oligomeric Golgi Complex Subunit 2 J. Biol. Chem., August 10, 2007; 282(32): 23418 - 23426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Foulquier, D. Ungar, E. Reynders, R. Zeevaert, P. Mills, M. T. Garcia-Silva, P. Briones, B. Winchester, W. Morelle, M. Krieger, et al. A new inborn error of glycosylation due to a Cog8 deficiency reveals a critical role for the Cog1-Cog8 interaction in COG complex formation Hum. Mol. Genet., April 1, 2007; 16(7): 717 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kranz, B. G. Ng, L. Sun, V. Sharma, E. A. Eklund, Y. Miura, D. Ungar, V. Lupashin, R. D. Winkel, J. F. Cipollo, et al. COG8 deficiency causes new congenital disorder of glycosylation type IIh Hum. Mol. Genet., April 1, 2007; 16(7): 731 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wopereis, S. Grunewald, K. M.L.C. Huijben, E. Morava, R. Mollicone, B. G.M. van Engelen, D. J. Lefeber, and R. A. Wevers Transferrin and Apolipoprotein C-III Isofocusing Are Complementary in the Diagnosis of N- and O-Glycan Biosynthesis Defects Clin. Chem., February 1, 2007; 53(2): 180 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. Klyachkin, K. D. Stoops, and R. J. Geraghty Herpes simplex virus type 1 glycoprotein L mutants that fail to promote trafficking of glycoprotein H and fail to function in fusion can induce binding of glycoprotein L-dependent anti-glycoprotein H antibodies. J. Gen. Virol., April 1, 2006; 87(Pt 4): 759 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wopereis, D. J. Lefeber, E. Morava, and R. A. Wevers Mechanisms in Protein O-Glycan Biosynthesis and Clinical and Molecular Aspects of Protein O-Glycan Biosynthesis Defects: A Review Clin. Chem., April 1, 2006; 52(4): 574 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Foulquier, E. Vasile, E. Schollen, N. Callewaert, T. Raemaekers, D. Quelhas, J. Jaeken, P. Mills, B. Winchester, M. Krieger, et al. Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II. PNAS, March 7, 2006; 103(10): 3764 - 3769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kubota, M. Sano, S. Goda, N. Suzuki, and K. Nishiwaki The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans Development, January 15, 2006; 133(2): 263 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ungar, T. Oka, E. Vasile, M. Krieger, and F. M. Hughson Subunit Architecture of the Conserved Oligomeric Golgi Complex J. Biol. Chem., September 23, 2005; 280(38): 32729 - 32735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, E. Vasile, M. Penman, C. D. Novina, D. M. Dykxhoorn, D. Ungar, F. M. Hughson, and M. Krieger Genetic Analysis of the Subunit Organization and Function of the Conserved Oligomeric Golgi (COG) Complex: STUDIES OF COG5- AND COG7-DEFICIENT MAMMALIAN CELLS J. Biol. Chem., September 23, 2005; 280(38): 32736 - 32745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fotso, Y. Koryakina, O. Pavliv, A. B. Tsiomenko, and V. V. Lupashin Cog1p Plays a Central Role in the Organization of the Yeast Conserved Oligomeric Golgi Complex J. Biol. Chem., July 29, 2005; 280(30): 27613 - 27623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Zolov and V. V. Lupashin Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells J. Cell Biol., February 28, 2005; 168(5): 747 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oka and M. Krieger Multi-Component Protein Complexes and Golgi Membrane Trafficking J. Biochem., February 1, 2005; 137(2): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
