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Originally published as MBC in Press, 10.1091/mbc.E04-01-0013 on April 16, 2004

Vol. 15, Issue 7, 3005-3014, July 2004

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The Drosophila tctex-1 Light Chain Is Dispensable for Essential Cytoplasmic Dynein Functions but Is Required during Spermatid Differentiation

Min-gang Li *, Madeline Serr *, Eric A. Newman {dagger}, and Thomas S. Hays * {ddagger}

* Department of Genetics, Cell, and Developmental Biology, University of Minnesota, St. Paul, Minnesota 55108; {dagger} Department of Neuroscience, University of Minnesota, St. Paul, Minnesota 55108

Submitted January 8, 2004; Revised April 7, 2004; Accepted April 8, 2004
Monitoring Editor: David Drubin

Variations in subunit composition and modification have been proposed to regulate the multiple functions of cytoplasmic dynein. Here, we examine the role of the Drosophila ortholog of tctex-1, the 14-kDa dynein light chain. We show that the 14-kDa light chain is a bona fide component of Drosophila cytoplasmic dynein and use P element excision to generate flies that completely lack this dynein subunit. Remarkably, the null mutant is viable and the only observed defect is complete male sterility. During spermatid differentiation, the 14-kDa light chain is required for the localization of a nuclear "cap" of cytoplasmic dynein and for proper attachment between the sperm nucleus and flagellar basal body. Our results provide evidence that the function of the 14-kDa light chain in Drosophila is distinct from other dynein subunits and is not required for any essential functions in early development or in the adult organism.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E04-01-0013. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-01-0013.

Online version of this article contains supporting material. Online version is available at www.molbiolcell.org.

{ddagger} Corresponding author. E-mail address: tom-h{at}biosci.cbs.umn.edu.




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