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Originally published as MBC in Press, 10.1091/mbc.E04-03-0222 on May 7, 2004

Vol. 15, Issue 7, 3224-3232, July 2004

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Na,K-ATPase {beta}1-Subunit Increases the Translation Efficiency of the {alpha}1-Subunit in MSV-MDCK Cells

Sigrid A. Rajasekaran *, Jegan Gopal *, Dianna Willis {dagger}, Cromwell Espineda *, Jeffery L. Twiss {dagger}, and Ayyappan K. Rajasekaran * {ddagger}

* Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California, Los Angeles, Los Angeles, California 90095; {dagger} Nemours Biomedical Research, Alfred I duPont Hospital for Children, Wilmington, Delaware 19803

Submitted March 16, 2004; Accepted April 27, 2004
Monitoring Editor: Marvin P. Wickens

The Na,K-ATPase consists of an {alpha}- and {beta}-subunit. Moloney sarcoma virus-transformed MDCK cells (MSV-MDCK) express low levels of Na,K-ATPase {beta}1-subunit. Ectopic expression of Na,K-ATPase {beta}1-subunit in these cells increased the protein levels of the {alpha}1-subunit of Na,K-ATPase. This increase was not due to altered transcription of the {alpha}1-subunit gene or half-life of the {alpha}1-subunit protein because both {alpha}1-subunit mRNA levels and half-life of the {alpha}1-subunit protein were comparable in MSV-MDCK and {beta}1-subunit expressing MSV-MDCK cells. However, short pulse labeling revealed that the initial translation rate of the {alpha}1-subunit in {beta}1-subunit expressing MSV-MDCK cells was six- to sevenfold higher compared with MSV-MDCK cells. The increased translation was specific to {alpha}1-subunit because translation rates of occludin and {beta}-catenin, membrane and cytosolic proteins, respectively, were not altered. In vitro cotranslation/translocation experiments using rabbit reticulocyte lysate and rough microsomes revealed that the {alpha}1-subunit mRNA is more efficiently translated in the presence of {beta}1-subunit. Furthermore, sucrose density gradient analysis revealed significantly more {alpha}1-subunit transcript associated with the polysomal fraction in {beta}1-subunit expressing MSV-MDCK cells compared with MSV-MDCK cells, indicating that in mammalian cells the Na,K-ATPase {beta}1-subunit is involved in facilitating the translation of the {alpha}1-subunit mRNA in the endoplasmic reticulum.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E04-03-0222. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-03-0222.

{ddagger} Corresponding author. E-mail address: arajasekaran{at}mednet.ucla.edu.




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