QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 15, Issue 8, 3863-3875, August 2004

This Article Full Text Full Text (PDF) All Versions of this Article: E03-08-0584v1 15/8/3863    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brederlau, A. Articles by Funa, K. Search for Related Content PubMed PubMed Citation Articles by Brederlau, A. Articles by Funa, K.

The Bone Morphogenetic Protein Type Ib Receptor Is a Major Mediator of Glial Differentiation and Cell Survival in Adult Hippocampal Progenitor Cell Culture

A. Brederlau ^* , R. Faigle ^* , M. Elmi ^* , A. Zarebski ^* , S. Sjöberg ^*, M. Fujii ^||, K. Miyazono ^||, and K. Funa ^*

^* Institute of Anatomy and Cell Biology, Göteborg University, SE-405 30 Gothenburg, Sweden; Jagiellonian University, Faculty of Biotechnology, Biocenter, 30-387 Krakow, Poland; and ^|| Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

Submitted August 12, 2003; Revised May 31, 2004; Accepted June 2, 2004
Monitoring Editor: Carl-Henrik Heldin

Bone morphogenetic proteins (BMPs) act as growth regulators and inducers of differentiation. They transduce their signal via three different type I receptors, termed activin receptor-like kinase 2 (Alk2), Alk3, or bone morphogenetic protein receptor Ia (BMPRIa) and Alk6 or BMPRIb. Little is known about functional differences between the three type I receptors. Here, we have investigated consequences of constitutively active (ca) and dominant negative (dn) type I receptor overexpression in adult-derived hippocampal progenitor cells (AHPs). The dn receptors have a nonfunctional intracellular but functional extracellular domain. They thus trap BMPs that are endogenously produced by AHPs. We found that effects obtained by overexpression of dnAlk2 and dnAlk6 were similar, suggesting similar ligand binding patterns for these receptors. Thus, cell survival was decreased, glial fibrillary acidic protein (GFAP) expression was reduced, whereas the number of oligodendrocytes increased. No effect on neuronal differentiation was seen. Whereas the expression of Alk2 and Alk3 mRNA remained unchanged, the Alk6 mRNA was induced after impaired BMP signaling. After dnAlk3 overexpression, cell survival and astroglial differentiation increased in parallel to augmented Alk6 receptor signaling. We conclude that endogenous BMPs mediate cell survival, astroglial differentiation and the suppression of oligodendrocytic cell fate mainly via the Alk6 receptor in AHP culture.

These authors were equally contributing second authors.

Corresponding author. E-mail address: anke.brederlau{at}anatcell.gu.se.

This article has been cited by other articles:

				K.-i. Inoue, K. Ito, M. Osato, B. Lee, S.-C. Bae, and Y. Ito The Transcription Factor Runx3 Represses the Neurotrophin Receptor TrkB during Lineage Commitment of Dorsal Root Ganglion Neurons J. Biol. Chem., August 17, 2007; 282(33): 24175 - 24184. [Abstract] [Full Text] [PDF]

				J. Samanta, G. M. Burke, T. McGuire, A. J. Pisarek, A. Mukhopadhyay, Y. Mishina, and J. A. Kessler BMPR1a Signaling Determines Numbers of Oligodendrocytes and Calbindin-Expressing Interneurons in the Cortex J. Neurosci., July 11, 2007; 27(28): 7397 - 7407. [Abstract] [Full Text] [PDF]

				I. Z. Jaffe, Y. Tintut, B. G. Newfell, L. L. Demer, and M. E. Mendelsohn Mineralocorticoid Receptor Activation Promotes Vascular Cell Calcification Arterioscler. Thromb. Vasc. Biol., April 1, 2007; 27(4): 799 - 805. [Abstract] [Full Text] [PDF]