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Vol. 15, Issue 9, 4064-4072, September 2004
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* Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5;
Departments of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5; and
Friedrich Miescher Laboratorium, Max-Planck-Gesellschaft, D-72076 Tübingen, Germany
Submitted April 15, 2004;
Revised June 25, 2004;
Accepted July 1, 2004
Monitoring Editor: Howard Riezman
The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEF) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the endoplasmic reticulum. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the ArfGAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.
Corresponding author. E-mail address: anne.spang{at}tuebingen.mpg.de.
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