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Vol. 15, Issue 9, 4261-4277, September 2004
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* Department of Cell and Molecular Biology, Karolinska Institutet, S-17177 Stockholm, Sweden;
Peptide Specialty Laboratories GmbH, D-69120 Heidelberg, Germany
Submitted March 1, 2004;
Accepted June 14, 2004
Monitoring Editor: Pamela Silver
The vertebrate nuclear pore complex (NPC) is a macromolecular assembly of protein subcomplexes forming a structure of eightfold radial symmetry. The NPC core consists of globular subunits sandwiched between two coaxial ring-like structures of which the ring facing the nuclear interior is capped by a fibrous structure called the nuclear basket. By postembedding immunoelectron microscopy, we have mapped the positions of several human NPC proteins relative to the NPC core and its associated basket, including Nup93, Nup96, Nup98, Nup107, Nup153, Nup205, and the coiled coil-dominated 267-kDa protein Tpr. To further assess their contributions to NPC and basket architecture, the genes encoding Nup93, Nup96, Nup107, and Nup205 were posttranscriptionally silenced by RNA interference (RNAi) in HeLa cells, complementing recent RNAi experiments on Nup153 and Tpr. We show that Nup96 and Nup107 are core elements of the NPC proper that are essential for NPC assembly and docking of Nup153 and Tpr to the NPC. Nup93 and Nup205 are other NPC core elements that are important for long-term maintenance of NPCs but initially dispensable for the anchoring of Nup153 and Tpr. Immunogold-labeling for Nup98 also results in preferential labeling of NPC core regions, whereas Nup153 is shown to bind via its amino-terminal domain to the nuclear coaxial ring linking the NPC core structures and Tpr. The position of Tpr in turn is shown to coincide with that of the nuclear basket, with different Tpr protein domains corresponding to distinct basket segments. We propose a model in which Tpr constitutes the central architectural element that forms the scaffold of the nuclear basket.
Abbreviations used: aa, amino acid(s); FA, formaldehyde; GA, glutaraldehyde; IFM, immunofluorescence microscopy; mAb, monoclonal antibody; NE, nuclear envelope; NBD, nuclear pore complex binding domain; NPC, nuclear pore complex; Nup, nucleoporin; RNAi, RNA interference; siRNA, small interfering RNA.
Online version of this article contains supporting material. Online version is available at www.molbiolcell.org.
These authors contributed equally to this work.
Present address: Zentrum für Molekulare Biologie Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
|| Corresponding author. E-mail address: volker.cordes{at}cmb.ki.se.
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