![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, Issue 1, 248-259, January 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Institut für Genetik and Zentrum für Molekulare Medizin, Universität zu Köln, 50674 Köln, Germany;
Max-Planck-Institut für Biophysik, Abteilung für Strukturbiologie, 60439 Frankfurt am Main, Germany
Submitted September 15, 2004;
Revised October 21, 2004;
Accepted October 26, 2004
Monitoring Editor: Janet Shaw
Prohibitins comprise a remarkably conserved protein family in eukaryotic cells with proposed functions in cell cycle progression, senescence, apoptosis, and the regulation of mitochondrial activities. Two prohibitin homologues, Phb1 and Phb2, assemble into a high molecular weight complex of 
1.2 MDa in the mitochondrial inner membrane, but a nuclear localization of Phb1 and Phb2 also has been reported. Here, we have analyzed the biogenesis and structure of the prohibitin complex in Saccharomyces cerevisiae. Both Phb1 and Phb2 subunits are targeted to mitochondria by unconventional noncleavable targeting sequences at their amino terminal end. Membrane insertion involves binding of newly imported Phb1 to Tim8/13 complexes in the intermembrane space and is mediated by the TIM23-translocase. Assembly occurs via intermediate-sized complexes of 120 kDa containing both Phb1 and Phb2. Conserved carboxy-terminal coiled-coil regions in both subunits mediate the formation of large assemblies in the inner membrane. Single particle electron microscopy of purified prohibitin complexes identifies diverse ring-shaped structures with outer dimensions of 270 x 200 Å. Implications of these findings for proposed cellular activities of prohibitins are discussed.
Corresponding author. E-mail address: thomas.langer{at}unikoeln.de.
This article has been cited by other articles:
|
|
 |
|
  V. Emerson, D. Holtkotte, T. Pfeiffer, I-H. Wang, M. Schnolzer, T. Kempf, and V. Bosch Identification of the Cellular Prohibitin 1/Prohibitin 2 Heterodimer as an Interaction Partner of the C-Terminal Cytoplasmic Domain of the HIV-1 Glycoprotein J. Virol., February 1, 2010; 84(3): 1355 - 1365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Osman, C. Merkwirth, and T. Langer Prohibitins and the functional compartmentalization of mitochondrial membranes J. Cell Sci., November 1, 2009; 122(21): 3823 - 3830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Boehm, J. Nield, P. Zhang, E.-M. Aro, J. Komenda, and P. J. Nixon Structural and Mutational Analysis of Band 7 Proteins in the Cyanobacterium Synechocystis sp. Strain PCC 6803 J. Bacteriol., October 15, 2009; 191(20): 6425 - 6435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Akiyama Quality Control of Cytoplasmic Membrane Proteins in Escherichia coli J. Biochem., October 1, 2009; 146(4): 449 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tatsuta Protein Quality Control in Mitochondria J. Biochem., October 1, 2009; 146(4): 455 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. P. Pearce, D. B. Wormer, S. Wilkens, and R. J. H. Wojcikiewicz An Endoplasmic Reticulum (ER) Membrane Complex Composed of SPFH1 and SPFH2 Mediates the ER-associated Degradation of Inositol 1,4,5-Trisphosphate Receptors J. Biol. Chem., April 17, 2009; 284(16): 10433 - 10445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Hoegg, D. T. Browman, M. E. Resek, and S. M. Robbins Distinct Regions within the Erlins Are Required for Oligomerization and Association with High Molecular Weight Complexes J. Biol. Chem., March 20, 2009; 284(12): 7766 - 7776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Osman, M. Haag, C. Potting, J. Rodenfels, P. V. Dip, F. T. Wieland, B. Brugger, B. Westermann, and T. Langer The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria J. Cell Biol., February 23, 2009; 184(4): 583 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iacovino, C. Granycome, H. Sembongi, M. Bokori-Brown, R. A. Butow, I. J. Holt, and J. M. Bateman The conserved translocase Tim17 prevents mitochondrial DNA loss Hum. Mol. Genet., January 1, 2009; 18(1): 65 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wagner, N. Gebert, B. Guiard, K. Brandner, K. N. Truscott, N. Wiedemann, N. Pfanner, and P. Rehling The Assembly Pathway of the Mitochondrial Carrier Translocase Involves Four Preprotein Translocases Mol. Cell. Biol., July 1, 2008; 28(13): 4251 - 4260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Ross, Z. S. Nagy, and R. A. Kirken The PHB1/2 Phosphocomplex Is Required for Mitochondrial Homeostasis and Survival of Human T Cells J. Biol. Chem., February 22, 2008; 283(8): 4699 - 4713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Merkwirth, S. Dargazanli, T. Tatsuta, S. Geimer, B. Lower, F. T. Wunderlich, J.-C. von Kleist-Retzow, A. Waisman, B. Westermann, and T. Langer Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria Genes & Dev., February 15, 2008; 22(4): 476 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. He, Q. Feng, A. Mukherjee, D. M. Lonard, F. J. DeMayo, B. S. Katzenellenbogen, J. P. Lydon, and B. W. O'Malley A Repressive Role for Prohibitin in Estrogen Signaling Mol. Endocrinol., February 1, 2008; 22(2): 344 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schleicher, B. R. Shepherd, Y. Suarez, C. Fernandez-Hernando, J. Yu, Y. Pan, L. M. Acevedo, G. S. Shadel, and W. C. Sessa Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence J. Cell Biol., January 10, 2008; 180(1): 101 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Marques, N. A. Dencher, A. Videira, and F. Krause Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria Eukaryot. Cell, December 1, 2007; 6(12): 2391 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. P. Pearce, Y. Wang, G. G. Kelley, and R. J. H. Wojcikiewicz SPFH2 Mediates the Endoplasmic Reticulum-associated Degradation of Inositol 1,4,5-Trisphosphate Receptors and Other Substrates in Mammalian Cells J. Biol. Chem., July 13, 2007; 282(28): 20104 - 20115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hajek, A. Chomyn, and G. Attardi Identification of a Novel Mitochondrial Complex Containing Mitofusin 2 and Stomatin-like Protein 2 J. Biol. Chem., February 23, 2007; 282(8): 5670 - 5681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Osman, C. Wilmes, T. Tatsuta, and T. Langer Prohibitins Interact Genetically with Atp23, a Novel Processing Peptidase and Chaperone for the F1FO-ATP Synthase Mol. Biol. Cell, February 1, 2007; 18(2): 627 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Theiss, R. D. Idell, S. Srinivasan, J.-M. Klapproth, D. P. Jones, D. Merlin, and S. V. Sitaraman Prohibitin protects against oxidative stress in intestinal epithelial cells FASEB J, January 1, 2007; 21(1): 197 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kasashima, E. Ohta, Y. Kagawa, and H. Endo Mitochondrial Functions and Estrogen Receptor-dependent Nuclear Translocation of Pleiotropic Human Prohibitin 2 J. Biol. Chem., November 24, 2006; 281(47): 36401 - 36410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Zubovych, T. Doundoulakis, P. G. Harran, and M. G. Roth A missense mutation in Caenorhabditis elegans prohibitin 2 confers an atypical multidrug resistance PNAS, October 17, 2006; 103(42): 15523 - 15528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Umlauf, M. Mairhofer, and R. Prohaska Characterization of the Stomatin Domain Involved in Homo-oligomerization and Lipid Raft Association J. Biol. Chem., August 18, 2006; 281(33): 23349 - 23356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER J. Cell Sci., August 1, 2006; 119(15): 3149 - 3160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kambacheld, S. Augustin, T. Tatsuta, S. Muller, and T. Langer Role of the Novel Metallopeptidase MoP112 and Saccharolysin for the Complete Degradation of Proteins Residing in Different Subcompartments of Mitochondria J. Biol. Chem., May 20, 2005; 280(20): 20132 - 20139. [Abstract] [Full Text] [PDF]
|
|
