![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, Issue 1, 405-420, January 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Fundación Instituto Leloir, IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, IIBBACONICET, Buenos Aires, Argentina;
Instituto de Química y Fisicoquímica Biológica, Facultad de Farmacia y Bioquímica, University of Buenos Aires, CONICET, Buenos Aires, Argentina;
Department of Neurology, Instituto de Investigaciones Neurológicas Raul Carrea (FLENI), Buenos Aires, Argentina; and
|| Institute for Cell Biochemistry and Clinical Neurobiology, Center for Molecular Neurobiology, University Hospital Hamburg-Eppendorf, University of Hamburg, Hamburg, Germany
Submitted June 24, 2004;
Revised September 29, 2004;
Accepted October 20, 2004
Monitoring Editor: Peter Walter
Staufen is a conserved double-stranded RNA-binding protein required for mRNA localization in Drosophila oocytes and embryos. The mammalian homologues Staufen 1 and Staufen 2 have been implicated in dendritic RNA targeting in neurons. Here we show that in rodent oligodendrocytes, these two proteins are present in two independent sets of RNA granules located at the distal myelinating processes. A third kind of RNA granules lacks Staufen and contains major myelin mRNAs. Myelin Staufen granules associate with microfilaments and microtubules, and their subcellular distribution is affected by polysome-disrupting drugs. Under oxidative stress, both Staufen 1 and Staufen 2 are recruited into stress granules (SGs), which are stress-induced organelles containing transiently silenced messengers. Staufen SGs contain the poly(A)-binding protein (PABP), the RNA-binding proteins HuR and TIAR, and small but not large ribosomal subunits. Staufen recruitment into perinuclear SGs is paralleled by a similar change in the overall localization of polyadenylated RNA. Under the same conditions, the distribution of recently transcribed and exported mRNAs is not affected. Our results indicate that Staufen 1 and Staufen 2 are novel and ubiquitous SG components and suggest that Staufen RNPs are involved in repositioning of most polysomal mRNAs, but not of recently synthesized transcripts, during the stress response.
Abbreviations used: RNP, ribonucleoparticle; MBPs, myelin basic proteins; PABP, poly(A)-binding protein; SGs, stress granules; RBD, double-stranded RNA-binding domain; ER, endoplasmic reticulum; PDI, protein disulphide isomerase; DRB, 5,6-dichlobenzimidazole riboside; NMD, non-sense-mediated decay.
These authors contributed equally to this work.
¶ Corresponding author. E-mail address: gboccaccio{at}leloir.org.ar.
This article has been cited by other articles:
|
|
 |
|
  L. G. Abrahamyan, L. Chatel-Chaix, L. Ajamian, M. P. Milev, A. Monette, J.-F. Clement, R. Song, M. Lehmann, L. DesGroseillers, M. Laughrea, et al. Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA J. Cell Sci., February 1, 2010; 123(3): 369 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Loschi, C. C. Leishman, N. Berardone, and G. L. Boccaccio Dynein and kinesin regulate stress-granule and P-body dynamics J. Cell Sci., November 1, 2009; 122(21): 3973 - 3982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Thomas, L. J. M. Tosar, M. A. Desbats, C. C. Leishman, and G. L. Boccaccio Mammalian Staufen 1 is recruited to stress granules and impairs their assembly J. Cell Sci., February 15, 2009; 122(4): 563 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lebeau, M. Maher-Laporte, L. Topolnik, C. E. Laurent, W. Sossin, L. DesGroseillers, and J.-C. Lacaille Staufen1 Regulation of Protein Synthesis-Dependent Long-Term Potentiation and Synaptic Function in Hippocampal Pyramidal Cells Mol. Cell. Biol., May 1, 2008; 28(9): 2896 - 2907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Chang, R. F. Zaarour, S. Reck-Peterson, J. Rinn, R. H. Singer, M. Snyder, P. Novick, and M. S. Mooseker Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body RNA, March 1, 2008; 14(3): 491 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Katahira, T. Miki, K. Takano, M. Maruhashi, M. Uchikawa, T. Tachibana, and Y. Yoneda Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs Nucleic Acids Res., February 2, 2008; 36(2): 616 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Furic, M. Maher-Laporte, and L. DesGroseillers A genome-wide approach identifies distinct but overlapping subsets of cellular mRNAs associated with Staufen1- and Staufen2-containing ribonucleoprotein complexes RNA, February 1, 2008; 14(2): 324 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Solomon, Y. Xu, B. Wang, M. D. David, P. Schubert, D. Kennedy, and J. W. Schrader Distinct Structural Features ofCaprin-1 Mediate Its Interaction with G3BP-1 and Its Induction of Phosphorylation of Eukaryotic Translation Initiation Factor 2{alpha}, Entry to Cytoplasmic Stress Granules, and Selective Interaction with a Subset of mRNAs Mol. Cell. Biol., March 15, 2007; 27(6): 2324 - 2342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Stohr, M. Lederer, C. Reinke, S. Meyer, M. Hatzfeld, R. H. Singer, and S. Huttelmaier ZBP1 regulates mRNA stability during cellular stress J. Cell Biol., November 20, 2006; 175(4): 527 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jolly and S. C. Lakhotia Human sat III and Drosophila hsr{omega} transcripts: a common paradigm for regulation of nuclear RNA processing in stressed cells Nucleic Acids Res., November 14, 2006; 34(19): 5508 - 5514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Dang, N. Kedersha, W.-K. Low, D. Romo, M. Gorospe, R. Kaufman, P. Anderson, and J. O. Liu Eukaryotic Initiation Factor 2{alpha}-independent Pathway of Stress Granule Induction by the Natural Product Pateamine A J. Biol. Chem., October 27, 2006; 281(43): 32870 - 32878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Vessey, A. Vaccani, Y. Xie, R. Dahm, D. Karra, M. A. Kiebler, and P. Macchi Dendritic localization of the translational repressor Pumilio 2 and its contribution to dendritic stress granules. J. Neurosci., June 14, 2006; 26(24): 6496 - 6508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hosoda, F. Lejeune, and L. E. Maquat Evidence that Poly(A) Binding Protein C1 Binds Nuclear Pre-mRNA Poly(A) Tails Mol. Cell. Biol., April 15, 2006; 26(8): 3085 - 3097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Anderson and N. Kedersha RNA granules J. Cell Biol., March 13, 2006; 172(6): 803 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hofmann, M. Casella, M. Schnolzer, T. Schlechter, H. Spring, and W. W. Franke Identification of the Junctional Plaque Protein Plakophilin 3 in Cytoplasmic Particles Containing RNA-binding Proteins and the Recruitment of Plakophilins 1 and 3 to Stress Granules Mol. Biol. Cell, March 1, 2006; 17(3): 1388 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Goetze, F. Tuebing, Y. Xie, M. M. Dorostkar, S. Thomas, U. Pehl, S. Boehm, P. Macchi, and M. A. Kiebler The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis J. Cell Biol., January 17, 2006; 172(2): 221 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Baez and G. L. Boccaccio Mammalian Smaug Is a Translational Repressor That Forms Cytoplasmic Foci Similar to Stress Granules J. Biol. Chem., December 30, 2005; 280(52): 43131 - 43140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dugre-Brisson, G. Elvira, K. Boulay, L. Chatel-Chaix, A. J. Mouland, and L. DesGroseillers Interaction of Staufen1 with the 5' end of mRNA facilitates translation of these RNAs Nucleic Acids Res., August 26, 2005; 33(15): 4797 - 4812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kedersha, G. Stoecklin, M. Ayodele, P. Yacono, J. Lykke-Andersen, M. J. Fritzler, D. Scheuner, R. J. Kaufman, D. E. Golan, and P. Anderson Stress granules and processing bodies are dynamically linked sites of mRNP remodeling J. Cell Biol., June 20, 2005; 169(6): 871 - 884. [Abstract] [Full Text] [PDF]
|
|
