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Originally published as MBC in Press, 10.1091/mbc.E05-04-0305 on July 12, 2005

Vol. 16, Issue 10, 4485-4494, October 2005

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Induction of Cytokinesis Is Independent of Precisely Regulated Microtubule Dynamics{boxv}

Laila I. Strickland, Erin J. Donnelly, and David R. Burgess

Boston College, Chestnut Hill, MA 02467

Submitted April 12, 2005; Revised June 28, 2005; Accepted July 6, 2005
Monitoring Editor: Ted Salmon

Astral microtubules (MTs) emanating from the mitotic apparatus (MA) during anaphase are required for stimulation of cytokinesis in eggs. We have used green fluorescent protein-labeled EB1 to observe MT dynamics during mitosis and cytokinesis in normal sea urchin eggs. Analysis of astral MT growth rates during anaphase shows that MTs contact the polar cortex earlier than the equatorial cortex after anaphase onset but that a normal cleavage furrow is not induced until contact with MTs has been achieved throughout the cortex. To assess the role of MT dynamics in initiation of cytokinesis, we used a collection of small molecule drugs to affect dynamics. Hexylene glycol resulted in rapid astral elongation due to decreased MT catastrophe and precocious furrowing. Taxol suppressed MT dynamics but did not inhibit furrow induction when the MA was manipulated toward the cortex. Urethane resulted in short, highly dynamic astral MTs with increased catastrophe that also stimulated furrowing upon being brought into proximity to the cortex. Our findings indicate that astral MT contact with the cortex is necessary for furrow initiation but that the dynamic state of astral MTs does not affect their competency to stimulate furrowing.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05–04–0305) on July 12, 2005.

{boxv} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: David R. Burgess (david.burgess{at}bc.edu).




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