QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 10, 4867-4881, October 2005

This Article Full Text Full Text (PDF) Supplemental Movies All Versions of this Article: E04-12-1114v1 16/10/4867    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martín-Cuadrado, A. B. Articles by de Aldana, C. R. V. Search for Related Content PubMed PubMed Citation Articles by Martín-Cuadrado, A. B. Articles by de Aldana, C. R. V.

Role of Septins and the Exocyst Complex in the Function of Hydrolytic Enzymes Responsible for Fission Yeast Cell Separation

Ana Belén Martín-Cuadrado ^*, Jennifer L. Morrell , Mami Konomi , Hanbing An , Claudia Petit , Masako Osumi , Mohan Balasubramanian , Kathleen L. Gould , Francisco del Rey ^*, and Carlos R. Vázquez de Aldana ^*

^* Instituto de Microbiología Bioquímica, Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Cientificas/Universidad de Salamanca, 37007 Salamanca, Spain; Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37212; Laboratory of Electron Microscopy/Open Research Center, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan; and Laboratory of Cell Division, Temasek Life Sciences Laboratory, Singapore 117604, Singapore

Submitted December 23, 2004; Revised July 21, 2005; Accepted July 26, 2005
Monitoring Editor: Anthony Bretscher

Cell separation in Schizosaccharomyces pombe is achieved by the concerted action of the Eng1 endo--1,3-glucanase and the Agn1 endo--1,3-glucanase, which are transported to the septum and localize to a ringlike structure that surrounds the septum. The requirements for the correct localization of both hydrolases as a ring were analyzed using green fluorescent protein fusion proteins. Targeting to the septum required a functional exocyst, because both proteins failed to localize correctly in sec8-1 or exo70 mutants, suggesting that Agn1 and Eng1 might be two of the cargo proteins present in the vesicles that accumulate in exocyst mutants. Septins and Mid2 were also required for correct formation of a ring. In their absence, Eng1 and Agn1 were found in a disklike structure that spanned the septum, rather than in a ring. Even though septin and mid2 mutants have a cell separation defect, the septum and the distribution of linear -1,3-glucans were normal in these cells, suggesting that mislocalization of Eng1 and Agn1 might be the reason underlying the failure to separate efficiently. Thus, one of the functions of the septin ring would be to act as a positional marker for the localization of hydrolytic proteins to the medial region.

Abbreviations used: CAR, contractile actomyosin ring.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Carlos R. Vázquez de Aldana (cvazquez{at}usal.es).

This article has been cited by other articles:

				W. Ge and M. K. Balasubramanian Pxl1p, a Paxillin-related Protein, Stabilizes the Actomyosin Ring during Cytokinesis in Fission Yeast Mol. Biol. Cell, April 1, 2008; 19(4): 1680 - 1692. [Abstract] [Full Text] [PDF]

				A. Vjestica, X.-Z. Tang, and S. Oliferenko The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast Mol. Biol. Cell, March 1, 2008; 19(3): 1125 - 1138. [Abstract] [Full Text] [PDF]

				H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]

				A. Yoneda and T. L. Doering A Eukaryotic Capsular Polysaccharide Is Synthesized Intracellularly and Secreted via Exocytosis Mol. Biol. Cell, December 1, 2006; 17(12): 5131 - 5140. [Abstract] [Full Text] [PDF]

				R. Martin-Garcia and M.-H. Valdivieso The fission yeast Chs2 protein interacts with the type-II myosin Myo3p and is required for the integrity of the actomyosin ring J. Cell Sci., July 1, 2006; 119(13): 2768 - 2779. [Abstract] [Full Text] [PDF]

				M. Osumi, M. Konomi, T. Sugawara, T. Takagi, and M. Baba High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and low-voltage scanning electron microscopy and immunoelectron microscopy J. Electron Microsc. (Tokyo), April 1, 2006; 55(2): 75 - 88. [Abstract] [Full Text] [PDF]

				Q.-W. Jin, M. Zhou, A. Bimbo, M. K. Balasubramanian, and D. McCollum A Role for the Septation Initiation Network in Septum Assembly Revealed by Genetic Analysis of sid2-250 Suppressors Genetics, April 1, 2006; 172(4): 2101 - 2112. [Abstract] [Full Text] [PDF]

				C. S. Petit, S. Mehta, R. H. Roberts, and K. L. Gould Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression J. Cell Sci., December 15, 2005; 118(24): 5731 - 5742. [Abstract] [Full Text] [PDF]