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Vol. 16, Issue 11, 5191-5201, November 2005

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Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Hyphal Growth of the Fungus Ustilago maydis

Isabel Schuchardt ^*, Daniela Aßmann ^*, Eckhard Thines , Christian Schuberth , and Gero Steinberg ^*

^* Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Straße, D-35043 Marburg, Germany; Eckhard Thines, Institut für Biotechnologie und Wirkstoff-Forschung, TU Kaiserslautern, D-67663 Kaiserslautern, Germany; and Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

Submitted April 3, 2005; Revised August 12, 2005; Accepted August 16, 2005
Monitoring Editor: David Drubin

Long-distance transport is crucial for polar-growing cells, such as neurons and fungal hyphae. Kinesins and myosins participate in this process, but their functional interplay is poorly understood. Here, we investigate the role of kinesin motors in hyphal growth of the plant pathogen Ustilago maydis. Although the microtubule plus-ends are directed to the hyphal tip, of all 10 kinesins analyzed, only conventional kinesin (Kinesin-1) and Unc104/Kif1A-like kinesin (Kinesin-3) were up-regulated in hyphae and they are essential for extended hyphal growth. kin1 and kin3 mutant hyphae grew irregular and remained short, but they were still able to grow polarized. No additional phenotype was detected in kin1rkin3 double mutants, but polarity was lost in myo5rkin1 and myo5rkin3 mutant cells, suggesting that kinesins and class V myosin cooperate in hyphal growth. Consistent with such a role in secretion, fusion proteins of green fluorescent protein and Kinesin-1, Myosin-V, and Kinesin-3 accumulate in the apex of hyphae, a region where secretory vesicles cluster to form the fungal Spitzenkörper. Quantitative assays revealed a role of Kin3 in secretion of acid phosphatase, whereas Kin1 was not involved. Our data demonstrate that just two kinesins and at least one myosin support hyphal growth.

Abbreviations used: aa, amino acid; GFP, green fluorescent protein; MT, microtubule; ORF, open reading frame; RFP, red fluorescent protein.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Gero Steinberg (gero.steinberg{at}staff.uni-marburg.de).

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