![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, Issue 12, 5804-5818, December 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794-5215;
Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030; and
Department of Chromosome Research, University of Vienna, A-1030 Vienna, Austria
Submitted May 31, 2005;
Revised September 26, 2005;
Accepted September 29, 2005
Monitoring Editor: Orna Cohen-Fix
Meiotic recombination differs from mitotic recombination in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change in partner choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-specific proteins, Hop1 and Red1. HOP1 contains two functional domains, called the N and C domains. Analysis of a point mutation that specifically inactivates the C domain (hop1-K593A) reveals that the N domain is sufficient for Hop1 localization to chromosomes and for Red1 and Hop1 interactions. The C domain is needed for spore viability, for chromosome synapsis, and for preventing DMC1-independent DSB repair, indicating it plays a role in the BSCR. All of the hop1-K593A phenotypes can be bypassed by fusion of ectopic dimerization domains to Mek1, suggesting that the function of the C domain is to promote Mek1 dimerization. Hop1 is a DSB-dependent phosphoprotein, whose phosphorylation requires the presence of the C domain, but is independent of MEK1. These results suggest a model in which Hop1 phosphorylation in response to DSBs triggers dimerization of Mek1 via the Hop1 C domain, thereby enabling Mek1 to phosphorylate target proteins that prevent repair of DSBs by sister chromatids.
Abbreviations used: BSCR, barrier to sister chromatid repair; DSB, double-strand break; AE, axial element; SC, synaptonemal complex.
These authors contributed equally to this work.
Address correspondence to: Nancy M. Hollingsworth (nhollin{at}ms.cc.sunysb.edu).
This article has been cited by other articles:
|
|
 |
|
  H. Cartagena-Lirola, I. Guerini, N. Manfrini, G. Lucchini, and M. P. Longhese Role of the Saccharomyces cerevisiae Rad53 Checkpoint Kinase in Signaling Double-Strand Breaks during the Meiotic Cell Cycle Mol. Cell. Biol., July 15, 2008; 28(14): 4480 - 4493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Sheridan, X. Yu, R. Roth, J. E. Heuser, M. G. Sehorn, P. Sung, E. H. Egelman, and D. K. Bishop A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments Nucleic Acids Res., July 1, 2008; 36(12): 4057 - 4066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Busygina, M. G. Sehorn, I. Y. Shi, H. Tsubouchi, G. S. Roeder, and P. Sung Hed1 regulates Rad51-mediated recombination via a novel mechanism Genes & Dev., March 15, 2008; 22(6): 786 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wan, H. Niu, B. Futcher, C. Zhang, K. M. Shokat, S. J. Boulton, and N. M. Hollingsworth Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast Genes & Dev., February 1, 2008; 22(3): 386 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sanchez-Moran, J.-L. Santos, G. H. Jones, and F. C. H. Franklin ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis Genes & Dev., September 1, 2007; 21(17): 2220 - 2233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Niu, X. Li, E. Job, C. Park, D. Moazed, S. P. Gygi, and N. M. Hollingsworth Mek1 Kinase Is Regulated To Suppress Double-Strand Break Repair between Sister Chromatids during Budding Yeast Meiosis Mol. Cell. Biol., August 1, 2007; 27(15): 5456 - 5467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Smolikov, A. Eizinger, A. Hurlburt, E. Rogers, A. M. Villeneuve, and M. P. Colaiacovo Synapsis-Defective Mutants Reveal a Correlation Between Chromosome Conformation and the Mode of Double-Strand Break Repair During Caenorhabditis elegans Meiosis Genetics, August 1, 2007; 176(4): 2027 - 2033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Rubenstein and M. C. Schmidt Mechanisms Regulating the Protein Kinases of Saccharomyces cerevisiae Eukaryot. Cell, April 1, 2007; 6(4): 571 - 583. [Full Text] [PDF]
|
|
|
|
|
|
L. Wan, C. Zhang, K. M. Shokat, and N. M. Hollingsworth Chemical Inactivation of Cdc7 Kinase in Budding Yeast Results in a Reversible Arrest That Allows Efficient Cell Synchronization Prior to Meiotic Recombination Genetics, December 1, 2006; 174(4): 1767 - 1774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Wells, D. W. Pryce, A. Estreicher, J. Loidl, and R. J. McFarlane Linear Element-Independent Meiotic Recombination in Schizosaccharomyces pombe Genetics, November 1, 2006; 174(3): 1105 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Yandeau-Nelson, Y. Xia, J. Li, M. G. Neuffer, and P. S. Schnable Unequal Sister Chromatid and Homolog Recombination at a Tandem Duplication of the a1 Locus in Maize Genetics, August 1, 2006; 173(4): 2211 - 2226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sheridan and D. K. Bishop Red-Hed regulation: recombinase Rad51, though capable of playing the leading role, may be relegated to supporting Dmc1 in budding yeast meiosis. Genes & Dev., July 1, 2006; 20(13): 1685 - 1691. [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Lui, T. L. Peoples-Holst, J. Chang Mell, H.-Y. Wu, E. W. Dean, and S. M. Burgess Analysis of Close Stable Homolog Juxtaposition During Meiosis in Mutants of Saccharomyces cerevisiae Genetics, July 1, 2006; 173(3): 1207 - 1222. [Abstract] [Full Text] [PDF]
|
|
