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Vol. 16, Issue 12, 5843-5856, December 2005

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The Actin Cytoskeleton Is Required for Selective Types of Autophagy, but Not Nonspecific Autophagy, in the Yeast Saccharomyces cerevisiae

Fulvio Reggiori ^* , Iryna Monastyrska ^*, Takahiro Shintani, and Daniel J. Klionsky

Life Sciences Institute and Departments of Molecular, Cellular, and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109

Submitted July 13, 2005; Revised September 15, 2005; Accepted September 29, 2005
Monitoring Editor: Suresh Subramani

Autophagy is a catabolic multitask transport route that takes place in all eukaryotic cells. During starvation, cytoplasmic components are randomly sequestered into huge double-membrane vesicles called autophagosomes and delivered into the lysosome/vacuole where they are destroyed. Cells are able to modulate autophagy in response to their needs, and under certain circumstances, cargoes such as aberrant protein aggregates, organelles and bacteria can be selectively and exclusively incorporated into autophagosomes. In the yeast Saccharomyces cerevisiae, for example, double-membrane vesicles are used to transport the Ape1 protease into the vacuole, or for the elimination of superfluous peroxisomes. In the present study we reveal that in this organism, actin plays a role in these two types of selective autophagy but not in the nonselective, bulk process. In particular, we show that precursor Ape1 is not correctly recruited to the PAS, the putative site of double-membrane vesicle biogenesis, and superfluous peroxisomes are not degraded in a conditional actin mutant. These phenomena correlate with a defect in Atg9 trafficking from the mitochondria to the PAS.

^* These authors contributed equally to this work.

Present address: Department of Cell Biology, University Medical Centre Utrecht, 3584 CX Utrecht, The Netherlands.

Address correspondence to: Daniel J. Klionsky (klionsky{at}umich.edu).

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