![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, Issue 2, 483-496, February 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Molecular Biology, Graduate School of Science, Nagoya University and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Nagoya 464-8602, Japan;
Department of Molecular Cell and Development Biology, Sinsheimer Laboratories, Howard Hughes Medical Institute, University of California, Santa Cruz, Santa Cruz, CA 95064
Submitted July 4, 2004;
Accepted November 10, 2004
Monitoring Editor: Suzanne Pfeffer
Kinesin-1 is a heterotetramer composed of kinesin heavy chain (KHC) and kinesin light chain (KLC). The Caenorhabditis elegans genome has a single KHC, encoded by the unc-116 gene, and two KLCs, encoded by the klc-1 and klc-2 genes. We show here that UNC-116/KHC and KLC-2 form a complex orthologous to conventional kinesin-1. KLC-2 also binds UNC-16, the C. elegans JIP3/JSAP1 JNK-signaling scaffold protein, and the UNC-14 RUN domain protein. The localization of UNC-16 and UNC-14 depends on kinesin-1 (UNC-116 and KLC-2). Furthermore, mutations in unc-16, klc-2, unc-116, and unc-14 all alter the localization of cargos containing synaptic vesicle markers. Double mutant analysis is consistent with these four genes functioning in the same pathway. Our data support a model whereby UNC-16 and UNC-14 function together as kinesin-1 cargos and regulators for the transport or localization of synaptic vesicle components.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
These authors contributed equally to this work.
Present address: Howard Hughes Medical Institute, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.
Address correspondence to: Yishi Jin (Jin{at}biology.ucsc.edu) or Kunihiro Matsumoto (g44177a{at}nucc.cc.nagoya-tt.ac.jp).
This article has been cited by other articles:
|
|
 |
|
  H. Toda, H. Mochizuki, R. Flores III, R. Josowitz, T. B. Krasieva, V. J. LaMorte, E. Suzuki, J. G. Gindhart, K. Furukubo-Tokunaga, and T. Tomoda UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly Genes & Dev., December 1, 2008; 22(23): 3292 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Cai, P.-Y. Pan, and Z.-H. Sheng Syntabulin-Kinesin-1 Family Member 5B-Mediated Axonal Transport Contributes to Activity-Dependent Presynaptic Assembly J. Neurosci., July 4, 2007; 27(27): 7284 - 7296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Itoh, M. Satoh, E. Kanno, and M. Fukuda Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells, September 1, 2006; 11(9): 1023 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-i. Ogura and Y. Goshima The autophagy-related kinase UNC-51 and its binding partner UNC-14 regulate the subcellular localization of the Netrin receptor UNC-5 in Caenorhabditis elegans Development, September 1, 2006; 133(17): 3441 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Gindhart Towards an understanding of kinesin-1 dependent transport pathways through the study of protein-protein interactions Brief Funct Genomic Proteomic, March 1, 2006; 5(1): 74 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Boucrot, T. Henry, J.-P. Borg, J.-P. Gorvel, and S. Meresse The Intracellular Fate of Salmonella Depends on the Recruitment of Kinesin Science, May 20, 2005; 308(5725): 1174 - 1178. [Abstract] [Full Text] [PDF]
|
|
