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Vol. 16, Issue 2, 497-506, February 2005
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Department of Microbiology, University of Marburg and Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg, Germany
Submitted February 2, 2004;
Accepted November 9, 2004
Monitoring Editor: John Pringle
Polarized growth in filamentous fungi requires the integrity of the microtubule (MT) cytoskeleton. We found that growing MTs in Aspergillus nidulans merge at the center of fast growing tips and discovered that a kinesin motor protein, KipA, related to Tea2p of Schizosaccharomyces pombe, is required for this process. In a 
kipA strain, MT plus ends reach the tip but show continuous lateral movement. Hyphae lose directionality and grow in curves, apparently due to mislocalization of the vesicle supply center (Spitzenkörper) in the apex. Green fluorescent protein (GFP)-KipA accumulates at MT plus ends, whereas a KipA rigor mutant protein, GFP-KipAG223E, coated MTs evenly. These findings suggest that KipA requires its intrinsic motor activity to reach the MT plus end. Using KipA as an MT plus-end marker, we found bidirectional organization of MTs and determined the locations of microtubule organizing centers at nuclei, in the cytoplasm, and at septa.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
* Present address: Max-Planck-Institute for Structural Molecular Biology, Notkestr. 85, c/o DESY, Geb. 25b, D-22607 Hamburg, Germany;
Present address: Institute of Cell and Molecular Biology, University of Edinburgh, Michael Swann Building, The King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom;
Present address: Applied Microbiology, University of Karlsruhe, Hertzstrasse 16, D-76187 Karlsruhe, Germany.
Corresponding author. E-mail address: reinhard.fischer{at}bio.uni-karlsruhe.de.
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