Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
 QUICK SEARCH:   [advanced]


     


Originally published as MBC in Press, 10.1091/mbc.E04-09-0852 on November 17, 2004

Vol. 16, Issue 2, 519-531, February 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-09-0852v1
16/2/519    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by DeLuca, J. G.
Right arrow Articles by McEwen, B. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DeLuca, J. G.
Right arrow Articles by McEwen, B. F.

Hec1 and Nuf2 Are Core Components of the Kinetochore Outer Plate Essential for Organizing Microtubule Attachment Sites{boxv}

Jennifer G. DeLuca * {dagger}, Yimin Dong {ddagger}, Polla Hergert {ddagger}, Joshua Strauss {ddagger}, Jennifer M. Hickey *, E. D. Salmon *, and Bruce F. McEwen {ddagger}

* Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; {ddagger} Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201

Submitted September 29, 2004; Accepted November 9, 2004
Monitoring Editor: J. Richard McIntosh

A major goal in the study of vertebrate mitosis is to identify proteins that create the kinetochore-microtubule attachment site. Attachment sites within the kinetochore outer plate generate microtubule dependent forces for chromosome movement and regulate spindle checkpoint protein assembly at the kinetochore. The Ndc80 complex, comprised of Ndc80 (Hec1), Nuf2, Spc24, and Spc25, is essential for metaphase chromosome alignment and anaphase chromosome segregation. It has also been suggested to have roles in kinetochore microtubule formation, production of kinetochore tension, and the spindle checkpoint. Here we show that Nuf2 and Hec1 localize throughout the outer plate, and not the corona, of the vertebrate kinetochore. They are part of a stable "core" region whose assembly dynamics are distinct from other outer domain spindle checkpoint and motor proteins. Furthermore, Nuf2 and Hec1 are required for formation and/or maintenance of the outer plate structure itself. Fluorescence light microscopy, live cell imaging, and electron microscopy provide quantitative data demonstrating that Nuf2 and Hec1 are essential for normal kinetochore microtubule attachment. Our results indicate that Nuf2 and Hec1 are required for organization of stable microtubule plus-end binding sites in the outer plate that are needed for the sustained poleward forces required for biorientation at kinetochores.


Article published online ahead of print in MBC in Press on November 17, 2004 (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-09-0852).

{boxv} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

{dagger} Corresponding author. E-mail address: jgdeluca{at}email.unc.edu.




This article has been cited by other articles:


Home page
Mol. Biol. CellHome page
S. A. Ribeiro, J. C. Gatlin, Y. Dong, A. Joglekar, L. Cameron, D. F. Hudson, C. J. Farr, B. F. McEwen, E. D. Salmon, W. C. Earnshaw, et al.
Condensin Regulates the Stiffness of Vertebrate Centromeres
Mol. Biol. Cell, May 1, 2009; 20(9): 2371 - 2380.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
B. F. McEwen and Y. Dong
Releasing the spindle assembly checkpoint without tension
J. Cell Biol., February 9, 2009; 184(3): 355 - 356.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
A. Toso, J. R. Winter, A. J. Garrod, A. C. Amaro, P. Meraldi, and A. D. McAinsh
Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly
J. Cell Biol., February 9, 2009; 184(3): 365 - 372.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
Q. Xu, S. Zhu, W. Wang, X. Zhang, W. Old, N. Ahn, and X. Liu
Regulation of Kinetochore Recruitment of Two Essential Mitotic Spindle Checkpoint Proteins by Mps1 Phosphorylation
Mol. Biol. Cell, January 1, 2009; 20(1): 10 - 20.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
O. J. Marshall, A. T. Marshall, and K.H. A. Choo
Three-dimensional localization of CENP-A suggests a complex higher order structure of centromeric chromatin
J. Cell Biol., December 29, 2008; 183(7): 1193 - 1202.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
A. R. Barr and F. Gergely
MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics
Mol. Cell. Biol., December 1, 2008; 28(23): 7199 - 7211.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
G. Wu, X.-L. Qiu, L. Zhou, J. Zhu, R. Chamberlin, J. Lau, P.-L. Chen, and W.-H. Lee
Small Molecule Targeting the Hec1/Nek2 Mitotic Pathway Suppresses Tumor Cell Growth in Culture and in Animal
Cancer Res., October 15, 2008; 68(20): 8393 - 8399.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Y. Yang, F. Wu, T. Ward, F. Yan, Q. Wu, Z. Wang, T. McGlothen, W. Peng, T. You, M. Sun, et al.
Phosphorylation of HsMis13 by Aurora B Kinase Is Essential for Assembly of Functional Kinetochore
J. Biol. Chem., September 26, 2008; 283(39): 26726 - 26736.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
R. Gassmann, A. Essex, J.-S. Hu, P. S. Maddox, F. Motegi, A. Sugimoto, S. M. O'Rourke, B. Bowerman, I. McLeod, J. R. Yates III, et al.
A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex
Genes & Dev., September 1, 2008; 22(17): 2385 - 2399.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Zhu, F. Wang, F. Yan, P. Y. Yao, J. Du, X. Gao, X. Wang, Q. Wu, T. Ward, J. Li, et al.
Septin 7 Interacts with Centromere-associated Protein E and Is Required for Its Kinetochore Localization
J. Biol. Chem., July 4, 2008; 283(27): 18916 - 18925.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
S. Ishii, Y. Kurasawa, J. Wong, and L.-y. Yu-Lee
Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment
PNAS, March 18, 2008; 105(11): 4179 - 4184.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
I. M. Porter, S. E. McClelland, G. A. Khoudoli, C. J. Hunter, J. S. Andersen, A. D. McAinsh, J. J. Blow, and J. R. Swedlow
Bod1, a novel kinetochore protein required for chromosome biorientation
J. Cell Biol., October 22, 2007; 179(2): 187 - 197.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. Liu, X. Ding, J. Du, X. Cai, Y. Huang, T. Ward, A. Shaw, Y. Yang, R. Hu, C. Jin, et al.
Human NUF2 Interacts with Centromere-associated Protein E and Is Essential for a Stable Spindle Microtubule-Kinetochore Attachment
J. Biol. Chem., July 20, 2007; 282(29): 21415 - 21424.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
P. S. Maddox, F. Hyndman, J. Monen, K. Oegema, and A. Desai
Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin
J. Cell Biol., March 12, 2007; 176(6): 757 - 763.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
S.-T. Liu, J. B. Rattner, S. A. Jablonski, and T. J. Yen
Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells
J. Cell Biol., October 9, 2006; 175(1): 41 - 53.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
S. L. Kline, I. M. Cheeseman, T. Hori, T. Fukagawa, and A. Desai
The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
J. Cell Biol., April 10, 2006; 173(1): 9 - 17.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
L. M. Quarmby and M. R. Mahjoub
Caught Nek-ing: cilia and centrioles
J. Cell Sci., November 15, 2005; 118(22): 5161 - 5169.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
S. V. Holt, M. A. S. Vergnolle, D. Hussein, M. J. Wozniak, V. J. Allan, and S. S. Taylor
Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint
J. Cell Sci., October 15, 2005; 118(20): 4889 - 4900.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. Ciferri, J. De Luca, S. Monzani, K. J. Ferrari, D. Ristic, C. Wyman, H. Stark, J. Kilmartin, E. D. Salmon, and A. Musacchio
Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore
J. Biol. Chem., August 12, 2005; 280(32): 29088 - 29095.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
S. Saitoh, K. Ishii, Y. Kobayashi, and K. Takahashi
Spindle Checkpoint Signaling Requires the Mis6 Kinetochore Subcomplex, Which Interacts with Mad2 and Mitotic Spindles
Mol. Biol. Cell, August 1, 2005; 16(8): 3666 - 3677.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. R. Wei, P. K. Sorger, and S. C. Harrison
Molecular organization of the Ndc80 complex, an essential kinetochore component
PNAS, April 12, 2005; 102(15): 5363 - 5367.
[Abstract] [Full Text] [PDF]


Home page
Phil Trans R Soc BHome page
E.D Salmon, D Cimini, L.A Cameron, and J.G DeLuca
Merotelic kinetochores in mammalian tissue cells
Phil Trans R Soc B, March 29, 2005; 360(1455): 553 - 568.
[Abstract] [Full Text] [PDF]




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.