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Originally published as MBC in Press, 10.1091/mbc.E04-10-0876 on December 1, 2004

Vol. 16, Issue 2, 769-775, February 2005

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Dynamics of Relative Chromosome Position during the Cell Cycle{boxv}

Jeroen Essers *, Wiggert A. van Cappellen {dagger}, Arjan F. Theil *, Ellen van Drunen *, Nicolaas G.J. Jaspers *, Jan H.J. Hoeijmakers *, Claire Wyman * {ddagger}, Wim Vermeulen *, and Roland Kanaar * {ddagger} §

* Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands; {dagger} Department of Reproduction and Development, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands; and {ddagger} Department of Radiation Oncology, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands

Submitted October 7, 2004; Revised November 9, 2004; Accepted November 16, 2004
Monitoring Editor: Kerry Bloom

The position of chromosomal neighborhoods in living cells was followed using three different methods for marking chromosomal domains occupying arbitrary locations in the nucleus; photobleaching of GFP-labeled histone H2B, local UV-marked DNA, and photobleaching of fluorescently labeled DNA. All methods revealed that global chromosomal organization can be reestablished through one cell division from mother to daughters. By simultaneously monitoring cell cycle stage in the cells in which relative chromosomal domain positions were tracked, we observed that chromosomal neighborhood organization is apparently lost in the early G1 phase of the cell cycle. However, the daughter cells eventually regain the general chromosomal organization pattern of their mothers, suggesting an active mechanism could be at play to reestablish chromosomal neighborhoods.

Article published online ahead of print in MBC in Press on December 1, 2004 (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-10-0876).

{boxv} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

§ Corresponding author. E-mail address: r.kanaar{at}erasmusmc.nl.




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