![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, Issue 2, 881-890, February 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12180-3596;
Hospital for Special Surgery, New York, NY 10021; and
University of Nevada, Las Vegas, School of Dental Medicine, Las Vegas, NV 89106
Submitted August 12, 2004;
Accepted November 16, 2004
Monitoring Editor: Mark Ginsberg
The laminin family of proteins is critical for managing a variety of cellular activities including migration, adhesion, and differentiation. In bone, the roles of laminins in controlling osteogenic differentiation of human mesenchymal stem cells (hMSC) are unknown. We report here that laminin-5 is found in bone and expressed by hMSC. hMSC isolated from bone synthesize laminin-5 and adhere to exogenous laminin-5 through 
3
1 integrin. Adhesion to laminin-5 activates extracellular signal-related kinase (ERK) within 30 min and leads to phosphorylation of the osteogenic transcription factor Runx2/CBFA-1 within 8 d. Cells plated on laminin-5 for 16 d express increased levels of osteogenic marker genes, and those plated for 21 d deposit a mineralized matrix, indicative of osteogenic differentiation. Addition of the ERK inhibitor PD98059 mitigates these effects. We conclude that contact with laminin-5 is sufficient to activate ERK and to stimulate osteogenic differentiation in hMSC.
Abbreviations used: BMP, bone morphogenic protein; COLL-I, collagen-I; ELF, enzyme-linked fluorescence; ERK, extracellular-related kinase; FN, fibronectin; FTIR, Fourier transform infrared; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; hMSC, human mesenchymal stem cells; Ln, Laminin; MEK1, MAPK kinase; nd-blotto, 5% non-dairy creamer in PBS + 0.2% Tween 20; OS, osteogenic supplement; pERK, phosphorylated ERK; pRunx2, phospho-runx2/CBFA-1; RIPA, radioimmunoprecipitation assay; VN, vitronectin.
Corresponding author. E-mail address: ploppg{at}rpi.edu.
This article has been cited by other articles:
|
|
 |
|
  S. Yokoo, S. Yamagami, T. Shimada, T. Usui, T.-a. Sato, S. Amano, M. Araie, and J. Hamuro A Novel Isolation Technique of Progenitor Cells in Human Corneal Epithelium Using Non-Tissue Culture Dishes Stem Cells, July 1, 2008; 26(7): 1743 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. P. Daley, S. B. Peters, and M. Larsen Extracellular matrix dynamics in development and regenerative medicine J. Cell Sci., February 1, 2008; 121(3): 255 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. H. Fan, A. Au, K. Tamama, R. Littrell, L. B. Richardson, J. W. Wright, A. Wells, and L. G. Griffith Tethered Epidermal Growth Factor Provides a Survival Advantage to Mesenchymal Stem Cells Stem Cells, May 1, 2007; 25(5): 1241 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoberg, M. Rudert, T. Pap, G. Klein, S. Gay, and W. K Aicher Attachment to laminin-111 facilitates transforming growth factor {beta}-induced expression of matrix metalloproteinase-3 in synovial fibroblasts Ann Rheum Dis, April 1, 2007; 66(4): 446 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hashimoto, Y. Kariya, and K. Miyazaki Regulation of Proliferation and Chondrogenic Differentiation of Human Mesenchymal Stem Cells by Laminin-5 (Laminin-332) Stem Cells, November 1, 2006; 24(11): 2346 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hakuno, T. Takahashi, J. Lammerding, and R. T. Lee Focal Adhesion Kinase Signaling Regulates Cardiogenesis of Embryonic Stem Cells J. Biol. Chem., November 25, 2005; 280(47): 39534 - 39544. [Abstract] [Full Text] [PDF]
|
|
