Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E04-10-0939 on December 22, 2004

Vol. 16, Issue 3, 1095-1107, March 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E04-10-0939v1
16/3/1095    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guarguaglini, G.
Right arrow Articles by Nigg, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guarguaglini, G.
Right arrow Articles by Nigg, E. A.

The Forkhead-associated Domain Protein Cep170 Interacts with Polo-like Kinase 1 and Serves as a Marker for Mature Centrioles

Giulia Guarguaglini * {dagger}, Peter I. Duncan * {ddagger}, York D. Stierhof §, Tim Holmström * ||, Stefan Duensing ¶, and Erich A. Nigg *

* Department of Cell Biology, Max-Planck Institute for Biochemistry, D-82152, Martinsried, Germany; § Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, D-72076 Tübingen, Germany; and University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA 15213

Submitted October 28, 2004; Revised December 10, 2004; Accepted December 13, 2004
Monitoring Editor: Trisha Davis

We report the characterization of Cep170, a forkhead-associated (FHA) domain protein of previously unknown function. Cep170 was identified in a yeast two-hybrid screen for interactors of Polo-like kinase 1 (Plk1). In human cells, Cep170 is constantly expressed throughout the cell cycle but phosphorylated during mitosis. It interacts with Plk1 in vivo and can be phosphorylated by Plk1 in vitro, suggesting that it is a physiological substrate of this kinase. Both overexpression and small interfering RNA (siRNA)-mediated depletion studies suggest a role for Cep170 in microtuble organization and cell morphology. Cep170 associates with centrosomes during interphase and with spindle microtubules during mitosis. As shown by immunoelectron microscopy, Cep170 associates with subdistal appendages, typical of the mature mother centriole. Thus, anti-Cep170 antibodies stain only one centriole during G1, S, and early G2, but two centrioles during late G2 phase of the cell cycle. We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-10-0939) on December 22, 2004.

Abbreviations used: Cep170, centrosomal protein 170; FHA, forkhead-associated; HPV-16, human papillomavirus-16; HU, hydroxyurea; IF, immunofluorescence; IP, immunoprecipitation; MT, microtubule; PCM, pericentriolar material; Plk1, polo-like kinase 1; siRNA, small interfering RNA.

{dagger} Present address: Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche, c/o University of Rome "La Sapienza", 00185 Rome, Italy

{ddagger} Present address: Nutrition Department, Nestlé Research Center, CH-1000 Lausanne 26, Switzerland

|| Present address: Turku Centre for Biotechnology, FIN-20521, Turku, Finland

Address correspondence to: Giulia Guarguaglini (giulia.guarguaglini{at}uniroma1.it).




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. R. Farina, A. Tacconelli, L. Cappabianca, G. Cea, S. Panella, A. Chioda, A. Romanelli, C. Pedone, A. Gulino, and A. R. Mackay
The Alternative TrkAIII Splice Variant Targets the Centrosome and Promotes Genetic Instability
Mol. Cell. Biol., September 1, 2009; 29(17): 4812 - 4830.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Korzeniewski, L. Zheng, R. Cuevas, J. Parry, P. Chatterjee, B. Anderton, A. Duensing, K. Munger, and S. Duensing
Cullin 1 Functions as a Centrosomal Suppressor of Centriole Multiplication by Regulating Polo-like Kinase 4 Protein Levels
Cancer Res., August 15, 2009; 69(16): 6668 - 6675.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. L. Nguyen, M. E. McLaughlin-Drubin, and K. Munger
Delocalization of the Microtubule Motor Dynein from Mitotic Spindles by the Human Papillomavirus E7 Oncoprotein Is Not Sufficient for Induction of Multipolar Mitoses
Cancer Res., November 1, 2008; 68(21): 8715 - 8722.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. W. Krauss, J. R. Spence, S. Bahmanyar, A. I. M. Barth, M. M. Go, D. Czerwinski, and A. J. Meyer
Downregulation of Protein 4.1R, a Mature Centriole Protein, Disrupts Centrosomes, Alters Cell Cycle Progression, and Perturbs Mitotic Spindles and Anaphase
Mol. Cell. Biol., April 1, 2008; 28(7): 2283 - 2294.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Sakamoto, A. Uezu, S. Kawauchi, T. Kuramoto, K. Makino, K. Umeda, N. Araki, H. Baba, and H. Nakanishi
Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain.
Genes Cells, April 1, 2008; 13(4): 295 - 312.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. L. Nguyen, C. Eichwald, M. L. Nibert, and K. Munger
Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Centrosomal Component {gamma}-Tubulin
J. Virol., December 15, 2007; 81(24): 13533 - 13543.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Graser, Y.-D. Stierhof, and E. A. Nigg
Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion
J. Cell Sci., December 15, 2007; 120(24): 4321 - 4331.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Graser, Y.-D. Stierhof, S. B. Lavoie, O. S. Gassner, S. Lamla, M. Le Clech, and E. A. Nigg
Cep164, a novel centriole appendage protein required for primary cilium formation
J. Cell Biol., October 22, 2007; 179(2): 321 - 330.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Jeong, J. Lee, K. Kim, J. C. Yoo, and K. Rhee
Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization
J. Cell Sci., June 15, 2007; 120(12): 2106 - 2116.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N.-K. Soung, Y. H. Kang, K. Kim, K. Kamijo, H. Yoon, Y.-S. Seong, Y.-L. Kuo, T. Miki, S. R. Kim, R. Kuriyama, et al.
Requirement of hCenexin for Proper Mitotic Functions of Polo-Like Kinase 1 at the Centrosomes
Mol. Cell. Biol., November 15, 2006; 26(22): 8316 - 8335.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Yan, R. Habedanck, and E. A. Nigg
A Complex of Two Centrosomal Proteins, CAP350 and FOP, Cooperates with EB1 in Microtubule Anchoring
Mol. Biol. Cell, February 1, 2006; 17(2): 634 - 644.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.