QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 4, 1673-1683, April 2005

This Article Full Text Full Text (PDF) All Versions of this Article: E04-10-0873v1 16/4/1673    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heidtman, M. Articles by Barlowe, C. Search for Related Content PubMed PubMed Citation Articles by Heidtman, M. Articles by Barlowe, C.

Yos1p Is a Novel Subunit of the Yip1p–Yif1p Complex and Is Required for Transport between the Endoplasmic Reticulum and the Golgi Complex

Matthew Heidtman ^*, Catherine Z. Chen , Ruth N. Collins , and Charles Barlowe ^*

^* Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755; Department of Molecular Medicine, Cornell University, Veterinary Medical Center, Ithaca, NY 14853

Submitted October 7, 2004; Accepted January 10, 2005
Monitoring Editor: Benjamin Glick

Yeast Yip1p is a member of a conserved family of transmembrane proteins that interact with Rab GTPases. Previous studies also have indicated a role for Yip1p in the biogenesis of endoplasmic reticulum (ER)-derived COPII transport vesicles. In this report, we describe the identification and characterization of the uncharacterized open reading frame YER074W-A as a novel multicopy suppressor of the thermosensitive yip1-4 strain. We have termed this gene Yip One Suppressor 1 (YOS1). Yos1p is essential for growth and for function of the secretory pathway; depletion or inactivation of Yos1p blocks transport between the ER and the Golgi complex. YOS1 encodes an integral membrane protein of 87 amino acids that is conserved in eukaryotes. Yos1p localizes to ER and Golgi membranes and is efficiently packaged into ER-derived COPII transport vesicles. Yos1p associates with Yip1p and Yif1p, indicating Yos1p is a novel subunit of the Yip1p–Yif1p complex.

Address correspondence to: Charles Barlowe (barlowe{at}dartmouth.edu).

This article has been cited by other articles:

				Z.-B. Luo, D. Janz, X. Jiang, C. Gobel, H. Wildhagen, Y. Tan, H. Rennenberg, I. Feussner, and A. Polle Upgrading Root Physiology for Stress Tolerance by Ectomycorrhizas: Insights from Metabolite and Transcriptional Profiling into Reprogramming for Stress Anticipation Plant Physiology, December 1, 2009; 151(4): 1902 - 1917. [Abstract] [Full Text] [PDF]

				A. Lorente-Rodriguez, M. Heidtman, and C. Barlowe Multicopy suppressor analysis of thermosensitive YIP1 alleles implicates GOT1 in transport from the ER J. Cell Sci., May 15, 2009; 122(10): 1540 - 1550. [Abstract] [Full Text] [PDF]

				W.-J. Guo and T.-H. David Ho An Abscisic Acid-Induced Protein, HVA22, Inhibits Gibberellin-Mediated Programmed Cell Death in Cereal Aleurone Cells Plant Physiology, August 1, 2008; 147(4): 1710 - 1722. [Abstract] [Full Text] [PDF]

				C. A. Bue, C. M. Bentivoglio, and C. Barlowe Erv26p Directs Pro-Alkaline Phosphatase into Endoplasmic Reticulum-derived Coat Protein Complex II Transport Vesicles Mol. Biol. Cell, November 1, 2006; 17(11): 4780 - 4789. [Abstract] [Full Text] [PDF]

				N. Gottig, E. V. Elias, R. Quiroga, M. J. Nores, A. J. Solari, M. C. Touz, and H. D. Lujan Active and Passive Mechanisms Drive Secretory Granule Biogenesis during Differentiation of the Intestinal Parasite Giardia lamblia J. Biol. Chem., June 30, 2006; 281(26): 18156 - 18166. [Abstract] [Full Text] [PDF]

				J. P. Kastenmayer, L. Ni, A. Chu, L. E. Kitchen, W.-C. Au, H. Yang, C. D. Carter, D. Wheeler, R. W. Davis, J. D. Boeke, et al. Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae. Genome Res., March 1, 2006; 16(3): 365 - 373. [Abstract] [Full Text] [PDF]