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Vol. 16, Issue 4, 2003-2017, April 2005
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* Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain;
Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232; and
Department of Genetics, University of Debrecen, 4010 Debrecen, Hungary
Submitted June 2, 2004;
Revised January 18, 2005;
Accepted January 20, 2005
Monitoring Editor: Randy Schekman
Schizosaccharomyces pombe cells divide by medial fission through contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Here we describe the identification of seven genes (adg1+, adg2+, adg3+, cfh4+, agn1+, eng1+, and mid2+) whose expression is induced by the transcription factor Ace2p. The expression of all of these genes varied during the cell cycle, maximum transcription being observed during septation. At least three of these proteins (Eng1p, Agn1p, and Cfh4p) localize to a ring-like structure that surrounds the septum region during cell separation. Deletion of the previously uncharacterized genes was not lethal to the cells, but produced defects or delays in cell separation to different extents. Electron microscopic observation of mutant cells indicated that the most severe defect is found in eng1
agn1 cells, lacking the Eng1p endo-
-1,3-glucanase and the Agn1p endo-
-glucanase. The phenotype of this mutant closely resembled that of ace2 mutants, forming branched chains of cells. This suggests that these two proteins are the main activities required for cell separation to be completed.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Carlos R. Vázquez de Aldana (cvazquez{at}usal.es).
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