Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E04-09-0844 on February 23, 2005

Vol. 16, Issue 5, 2191-2206, May 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E04-09-0844v1
16/5/2191    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Myers, S. A.
Right arrow Articles by Chung, C. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Myers, S. A.
Right arrow Articles by Chung, C. Y.

A Dictyostelium Homologue of WASP Is Required for Polarized F-Actin Assembly during Chemotaxis

Scott A. Myers *, Ji W. Han *, Yoonsung Lee * {dagger}, Richard A. Firtel {ddagger}, and Chang Y. Chung * {ddagger}

* Department of Pharmacology, Vanderbilt University Medical Center, Nashville TN 37232-6600; {ddagger} Section of Cell and Developmental Biology, Division of Biological Sciences, Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0634

Submitted September 27, 2004; Revised February 11, 2005; Accepted February 15, 2005
Monitoring Editor: Martin A. Schwartz

The actin cytoskeleton controls the overall structure of cells and is highly polarized in chemotaxing cells, with F-actin assembled predominantly in the anterior leading edge and to a lesser degree in the cell's posterior. Wiskott-Aldrich syndrome protein (WASP) has emerged as a central player in controlling actin polymerization. We have investigated WASP function and its regulation in chemotaxing Dictyostelium cells and demonstrated the specific and essential role of WASP in organizing polarized F-actin assembly in chemotaxing cells. Cells expressing very low levels of WASP show reduced F-actin levels and significant defects in polarized F-actin assembly, resulting in an inability to establish axial polarity during chemotaxis. GFP-WASP preferentially localizes at the leading edge and uropod of chemotaxing cells and the B domain of WASP is required for the localization of WASP. We demonstrated that the B domain binds to PI(4,5)P2 and PI(3,4,5)P3 with similar affinities. The interaction between the B domain and PI(3,4,5)P3 plays an important role for the localization of WASP to the leading edge in chemotaxing cells. Our results suggest that the spatial and temporal control of WASP localization and activation is essential for the regulation of directional motility.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04–09–0844) on February 23, 2005.

{dagger} Present address: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.

Address correspondence to: Chang Y. Chung (chang.chung{at}vanderbilt.edu).




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. Y. Pollitt and R. H. Insall
WASP and SCAR/WAVE proteins: the drivers of actin assembly
J. Cell Sci., August 1, 2009; 122(15): 2575 - 2578.
[Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y. Tang and R. H. Gomer
A Protein with Similarity to PTEN Regulates Aggregation Territory Size by Decreasing Cyclic AMP Pulse Size during Dictyostelium discoideum Development
Eukaryot. Cell, October 1, 2008; 7(10): 1758 - 1770.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. M. Cooper, D. A. Bennin, and A. Huttenlocher
The PCH Family Member Proline-Serine-Threonine Phosphatase-interacting Protein 1 Targets to the Leukocyte Uropod and Regulates Directed Cell Migration
Mol. Biol. Cell, August 1, 2008; 19(8): 3180 - 3191.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Caracino, C. Jones, M. Compton, and C. L. Saxe III
The N-Terminus of Dictyostelium Scar Interacts with Abi and HSPC300 and Is Essential for Proper Regulation and Function
Mol. Biol. Cell, May 1, 2007; 18(5): 1609 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Szczur, H. Xu, S. Atkinson, Y. Zheng, and M.-D. Filippi
Rho GTPase CDC42 regulates directionality and random movement via distinct MAPK pathways in neutrophils
Blood, December 15, 2006; 108(13): 4205 - 4213.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Han, L. Leeper, F. Rivero, and C. Y. Chung
Role of RacC for the Regulation of WASP and Phosphatidylinositol 3-Kinase during Chemotaxis of Dictyostelium
J. Biol. Chem., November 17, 2006; 281(46): 35224 - 35234.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
B. P. Somesh, G. Vlahou, M. Iijima, R. H. Insall, P. Devreotes, and F. Rivero
RacG Regulates Morphology, Phagocytosis, and Chemotaxis
Eukaryot. Cell, October 1, 2006; 5(10): 1648 - 1663.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. A. Myers, L. R. Leeper, and C. Y. Chung
WASP-interacting Protein Is Important for Actin Filament Elongation and Prompt Pseudopod Formation in Response to a Dynamic Chemoattractant Gradient
Mol. Biol. Cell, October 1, 2006; 17(10): 4564 - 4575.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. M. Veltman and P. J.M. Van Haastert
Guanylyl Cyclase Protein and cGMP Product Independently Control Front and Back of Chemotaxing Dictyostelium Cells
Mol. Biol. Cell, September 1, 2006; 17(9): 3921 - 3929.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.