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Originally published as MBC in Press, 10.1091/mbc.E04-12-1044 on February 23, 2005

Vol. 16, Issue 5, 2234-2247, May 2005

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Lysophosphatidylcholine-induced Surface Redistribution Regulates Signaling of the Murine G Protein-coupled Receptor G2A{boxd}

Li Wang *, Caius G. Radu *, Li V. Yang {dagger}, Laurent A. Bentolila {ddagger}, Mireille Riedinger {dagger}, and Owen N. Witte * {dagger}

* Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095; {dagger} Howard Hughes Medical Institute/UCLA, UCLA, Los Angeles, CA 90095; and {ddagger} Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095

Submitted December 2, 2004; Revised February 10, 2005; Accepted February 11, 2005
Monitoring Editor: Sandra Schmid

Intracellular trafficking and spatial dynamics of membrane receptors critically regulate receptor function. Using microscopic and subcellular fractionation analysis, we studied the localization of the murine G protein-coupled receptor G2A (muG2A). Evaluating green fluorescent protein-tagged, exogenously expressed as well as the endogenous muG2A, we observed that this receptor was spontaneously internalized and accumulated in endosomal compartments, whereas its surface expression was enhanced and stabilized by lysophosphatidylcholine (LPC) treatment. Monensin, a general inhibitor of recycling pathways, blocked LPC-regulated surface localization of muG2A as well as muG2A-dependent extracellular signal-regulated kinase (ERK) activation and cell migration induced by LPC treatment. Mutation of the conserved DRY motif (R-> A) enhanced the surface expression of muG2A, resulting in its resistance to monensin inhibition of ERK activation. Our data suggest that intracellular sequestration and surface expression regulated by LPC, rather than direct agonistic activity control the signaling responses of murine G2A toward LPC.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04–12–1044) on February 23, 2005.

Abbreviations used: LPA, lysophosphatitic acid; LPC, lysophosphatidylcholine; S1P, sphingosine 1-phosphate; SPC, sphingosylphosphoryl-choline.

{boxd} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Owen N. Witte (owenw{at}microbio.ucla.edu).




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