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Vol. 16, Issue 5, 2325-2338, May 2005

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Dissociation of the Nuf2-Ndc80 Complex Releases Centromeres from the Spindle-Pole Body during Meiotic Prophase in Fission Yeast

Haruhiko Asakawa ^*, Aki Hayashi ^*, Tokuko Haraguchi ^* , and Yasushi Hiraoka ^* 

^* Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651-2492, Japan; Department of Biology, Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan

Submitted November 16, 2004; Accepted February 16, 2005
Monitoring Editor: Trisha Davis

In the fission yeast Schizosaccharomyces pombe, centromeres remain clustered at the spindle-pole body (SPB) during mitotic interphase. In contrast, during meiotic prophase centromeres dissociate from the SPB. Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf2-Ndc80 complex proteins (Nuf2, Ndc80, Spc24, and Spc25) disappear from the centromere in meiotic prophase when the centromeres are separated from the SPB. The centromere protein Mis12 also dissociates during meiotic prophase; however, Mis6 remains throughout meiosis. When cells are induced to meiosis by inactivation of Pat1 kinase (a key negative regulator of meiosis), centromeres remain associated with the SPB during meiotic prophase. However, inactivation of Nuf2 by a mutation causes the release of centromeres from the SPB in pat1 mutant cells, suggesting that the Nuf2-Ndc80 complex connects centromeres to the SPB. We further found that removal of the Nuf2-Ndc80 complex from the centromere and centromere-SPB dissociation are caused by mating pheromone signaling. Because pat1 mutant cells also show aberrant chromosome segregation in the first meiotic division and this aberration is compensated by mating pheromone signaling, dissociation of the Nuf2-Ndc80 complex may be associated with remodeling of the kinetochore for meiotic chromosome segregation.

Address correspondence to: Yasushi Hiraoka (yasushi{at}nict.go.jp).

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