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Originally published as MBC in Press, 10.1091/mbc.E04-06-0483 on March 23, 2005

Vol. 16, Issue 6, 2681-2693, June 2005

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Glycoprotein gp130 of Dictyostelium discoideum Influences Macropinocytosis and Adhesion{boxd}

Catherine P. Chia, Sujatha Gomathinayagam, Robert J. Schmaltz, and Laura K. Smoyer

School of Biological Sciences, University of Nebraska–Lincoln, Lincoln, NE 68588-0118

Submitted June 12, 2004; Revised March 2, 2005; Accepted March 15, 2005
Monitoring Editor: Peter Devreotes

Glycoprotein gp130, found on the plasma membrane of Dictyostelium discoideum amoebae, was postulated previously to play a role in phagocytosis. The gene for gp130 was cloned and when translated, yielded a 768 amino acid preproprotein of 85.3 kDa. It had nearly 40% similarity to the 138 kDa family of glycoproteins implicated in sexual cell fusion during macrocyst formation in D. discoideum. The difference between the calculated size and observed Mr of 130 kDa on protein gels likely was due to N-glycosylation that was confirmed by lectin blots. Consistent with its surface-exposure, an antibody raised against recombinant protein stained the plasma membrane of D. discoideum amoebae. Gp130 and its transcripts were high during axenic growth of cells, but relatively low during growth on bacteria. The gene for gp130 was disrupted and cell lines lacking the glycoprotein were efficient phagocytes, indicating that gp130 was dispensable for phagocytosis. Gp130-null cells were similar in size to parent DH1 cells, had enhanced macropinocytosis and grew faster to higher densities. They also exhibited weaker cell-substrate adhesion but displayed greater cell-cell cohesion. Collectively, the data indicated that gp130 influenced macropinocytosis and played a role in adhesion during vegetative growth.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04–06–0483) on March 23, 2005.

{boxd} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Catherine P. Chia (cchia{at}unlserve.unl.edu).






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