Molecular Biology of the Cell click for ASCB 2009 Annual Meeting page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E04-10-0941 on March 30, 2005

Vol. 16, Issue 6, 2809-2821, June 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E04-10-0941v1
16/6/2809    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krsmanovic, T.
Right arrow Articles by Kölling, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krsmanovic, T.
Right arrow Articles by Kölling, R.

Control of Ste6 Recycling by Ubiquitination in the Early Endocytic Pathway in Yeast

Tamara Krsmanovic, Agnes Pawelec, Tobias Sydor *, and Ralf Kölling

Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany

Submitted October 28, 2004; Revised March 21, 2005; Accepted March 23, 2005
Monitoring Editor: Sandra Schmid

We present evidence that ubiquitination controls sorting of the ABC-transporter Ste6 in the early endocytic pathway. The intracellular distribution of Ste6 variants with reduced ubiquitination was examined. In contrast to wild-type Ste6, which was mainly localized to internal structures, these variants accumulated at the cell surface in a polar manner. When endocytic recycling was blocked by Ypt6 inactivation, the ubiquitination deficient variants were trapped inside the cell. This indicates that the polar distribution is maintained dynamically through endocytic recycling and localized exocytosis ("kinetic polarization"). Ste6 does not appear to recycle through late endosomes, because recycling was not blocked in class E vps (vacuolar protein sorting) mutants ({Delta}vps4, {Delta}vps27), which are affected in late endosome function and in the retromer mutant {Delta}vps35. Instead, recycling was partially affected in the sorting nexin mutant {Delta}snx4, which serves as an indication that Ste6 recycles through early endosomes. Enhanced recycling of wild-type Ste6 was observed in class D vps mutants ({Delta}pep12, {Delta}vps8, and {Delta}vps21). The identification of putative recycling signals in Ste6 suggests that recycling is a signal-mediated process. Endocytic recycling and localized exocytosis could be important for Ste6 polarization during the mating process.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04–10–0941) on March 30, 2005.

* Present address: Institut für Zellbiologie, Universität Bonn, Ulrich-Haberland-Straße 61A, D-53121 Bonn, Germany.

Address correspondence to: Ralf Kölling (koelling{at}uni-hohenheim.de).




This article has been cited by other articles:


Home page
 MicrobiologyHome page
T. Iwaki, M. Onishi, M. Ikeuchi, A. Kita, R. Sugiura, Y. Giga-Hama, Y. Fukui, and K. Takegawa
Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
Microbiology, August 1, 2007; 153(8): 2753 - 2764.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Liu, Z. Hua, J. A. Nepute, and T. R. Graham
Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway
Mol. Biol. Cell, February 1, 2007; 18(2): 487 - 500.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.