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Vol. 16, Issue 6, 3052-3063, June 2005

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Mto2p, a Novel Fission Yeast Protein Required for Cytoplasmic Microtubule Organization and Anchoring of the Cytokinetic Actin Ring

Srinivas Venkatram ^*, Jennifer L. Jennings , Andrew Link , and Kathleen L. Gould ^*

^* Department of Cell and Developmental Biology and Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232; Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232

Submitted December 2, 2004; Revised March 8, 2005; Accepted March 17, 2005
Monitoring Editor: Kerry Bloom

Microtubules regulate diverse cellular processes, including chromosome segregation, nuclear positioning, and cytokinesis. In many organisms, microtubule nucleation requires -tubulin and associated proteins present at specific microtubule organizing centers (MTOCs). In fission yeast, interphase cytoplasmic microtubules originate from poorly characterized interphase MTOCs and spindle pole body (SPB), and during late anaphase from the equatorial MTOC (EMTOC). It has been previously shown that Mto1p (Mbo1p/Mod20p) function is important for the organization/nucleation of all cytoplasmic microtubules. Here, we show that Mto2p, a novel protein, interacts with Mto1p and is important for establishing a normal interphase cytoplasmic microtubule array. In addition, mto2 cells fail to establish a stable EMTOC and localize -tubulin complex members to this medial structure. As predicted from these functions, Mto2p localizes to microtubules, the SPB, and the EMTOC in an Mto1p-dependent manner. mto2 cells fail to anchor the cytokinetic actin ring in the medial region of the cell and under conditions that mildly perturb actin structures, these rings unravel in mto2 cells. Our results suggest that the Mto2p and the EMTOC are critical for anchoring the cytokinetic actin ring to the medial region of the cell and for proper coordination of mitosis with cytokinesis.

Abbreviations used: CAR, cytokinetic actin ring; EMTOC, equatorial microtubule organizing center; LatA, latrunculin A; MTOC, microtubule organizing center; PAA, postanaphase array; SPB, spindle pole body.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Kathleen L. Gould (kathy.gould{at}vanderbilt.edu).

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