QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 7, 3273-3288, July 2005

This Article Full Text Full Text (PDF) All Versions of this Article: E05-01-0060v1 16/7/3273    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hermann, G. J. Articles by Priess, J. R. Search for Related Content PubMed PubMed Citation Articles by Hermann, G. J. Articles by Priess, J. R.

Genetic Analysis of Lysosomal Trafficking in Caenorhabditis elegans

Greg J. Hermann ^*, Lena K. Schroeder ^*, Caroline A. Hieb ^*, Aaron M. Kershner ^*, Beverley M. Rabbitts ^*, Paul Fonarev , Barth D. Grant , and James R. Priess   ^||

^* Department of Biology, Lewis and Clark College, Portland, OR 97219; Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; Department of Zoology, University of Washington, Seattle, WA 98195; and ^|| Howard Hughes Medical Institute, Seattle, WA 98109

Submitted January 24, 2005; Revised April 5, 2005; Accepted April 8, 2005
Monitoring Editor: Sandra Schmid

The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.

Address correspondence to: Greg J. Hermann (hermann{at}lclark.edu).

This article has been cited by other articles:

				L. Nilsson, B. Conradt, A.-F. Ruaud, C. C.-H. Chen, J. Hatzold, J.-L. Bessereau, B. D. Grant, and S. Tuck Caenorhabditis elegans num-1 Negatively Regulates Endocytic Recycling Genetics, May 1, 2008; 179(1): 375 - 387. [Abstract] [Full Text] [PDF]

				E. Currie, B. King, A. L. Lawrenson, L. K. Schroeder, A. M. Kershner, and G. J. Hermann Role of the Caenorhabditis elegans Multidrug Resistance Gene, mrp-4, in Gut Granule Differentiation Genetics, November 1, 2007; 177(3): 1569 - 1582. [Abstract] [Full Text] [PDF]

				A. Holmes, A. Flett, D. Coudreuse, H. C. Korswagen, and J. Pettitt C. elegans Disabled is required for cell-type specific endocytosis and is essential in animals lacking the AP-3 adaptor complex J. Cell Sci., August 1, 2007; 120(15): 2741 - 2751. [Abstract] [Full Text] [PDF]

				L. K. Schroeder, S. Kremer, M. J. Kramer, E. Currie, E. Kwan, J. L. Watts, A. L. Lawrenson, and G. J. Hermann Function of the Caenorhabditis elegans ABC Transporter PGP-2 in the Biogenesis of a Lysosome-related Fat Storage Organelle Mol. Biol. Cell, March 1, 2007; 18(3): 995 - 1008. [Abstract] [Full Text] [PDF]

				N. de Souza, L. G. Vallier, H. Fares, and I. Greenwald SEL-2, the C. elegans neurobeachin/LRBA homolog, is a negative regulator of lin-12/Notch activity and affects endosomal traffic in polarized epithelial cells Development, February 15, 2007; 134(4): 691 - 702. [Abstract] [Full Text] [PDF]

				V. V. Captan, B. Goszczynski, and J. D. McGhee Neither Maternal nor Zygotic med-1/med-2 Genes Play a Major Role in Specifying the Caenorhabditis elegans Endoderm Genetics, February 1, 2007; 175(2): 969 - 974. [Abstract] [Full Text] [PDF]

				C. Wasmeier, M. Romao, L. Plowright, D. C. Bennett, G. Raposo, and M. C. Seabra Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes J. Cell Biol., October 23, 2006; 175(2): 271 - 281. [Abstract] [Full Text] [PDF]

				M. S. Marks Darkness descends with two Rabs J. Cell Biol., October 23, 2006; 175(2): 199 - 200. [Abstract] [Full Text] [PDF]

				M. Artal-Sanz, C. Samara, P. Syntichaki, and N. Tavernarakis Lysosomal biogenesis and function is critical for necrotic cell death in Caenorhabditis elegans J. Cell Biol., April 24, 2006; 173(2): 231 - 239. [Abstract] [Full Text] [PDF]

				C. C.-H. Chen, P. J. Schweinsberg, S. Vashist, D. P. Mareiniss, E. J. Lambie, and B. D. Grant RAB-10 Is Required for Endocytic Recycling in the Caenorhabditis elegans Intestine Mol. Biol. Cell, March 1, 2006; 17(3): 1286 - 1297. [Abstract] [Full Text] [PDF]