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Vol. 16, Issue 7, 3273-3288, July 2005
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* Department of Biology, Lewis and Clark College, Portland, OR 97219;
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854;
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
Department of Zoology, University of Washington, Seattle, WA 98195; and
|| Howard Hughes Medical Institute, Seattle, WA 98109
Submitted January 24, 2005;
Revised April 5, 2005;
Accepted April 8, 2005
Monitoring Editor: Sandra Schmid
The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.
Address correspondence to: Greg J. Hermann (hermann{at}lclark.edu).
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