QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 7, 3387-3400, July 2005

This Article Full Text Full Text (PDF) All Versions of this Article: E04-12-1081v1 16/7/3387    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bensen, E. S. Articles by Berman, J. Search for Related Content PubMed PubMed Citation Articles by Bensen, E. S. Articles by Berman, J.

The Mitotic Cyclins Clb2p and Clb4p Affect Morphogenesis in Candida albicans

Eric S. Bensen ^* , Andres Clemente-Blanco , Kenneth R. Finley ^*, Jaime Correa-Bordes , and Judith Berman ^* 

^* Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455; Department of Microbiology, University of Minnesota, Minneapolis, MN 55455; and Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain

Submitted December 14, 2004; Revised April 28, 2005; Accepted May 3, 2005
Monitoring Editor: Mark Solomon

The ability of Candida albicans to switch cellular morphologies is crucial for its ability to cause infection. Because the cell cycle machinery participates in Saccharomyces cerevisiae filamentous growth, we characterized in detail the two C. albicans B-type cyclins, CLB2 and CLB4, to better understand the molecular mechanisms that underlie the C. albicans morphogenic switch. Both Clb2p and Clb4p levels are cell cycle regulated, peaking at G2/M and declining before mitotic exit. On hyphal induction, the accumulation of the G1 cyclin Cln1p was prolonged, whereas the accumulation of both Clb proteins was delayed when compared with yeast form cells, indicating that CLB2 and CLB4 are differentially regulated in the two morphologies and that the dynamics of cyclin appearance differs between yeast and hyphal forms of growth. Clb2p-depleted cells were inviable and arrested with hyper-elongated projections containing two nuclei, suggesting that Clb2p is not required for entry into mitosis. Unlike Clb2p-depleted cells, Clb4p-depleted cells were viable and formed constitutive pseudohyphae. Clb proteins lacking destruction box domains blocked cell cycle progression resulting in the formation of long projections, indicating that both Clb2p and Clb4p must be degraded before mitotic exit. In addition, overexpression of either B-type cyclin reduced the extent of filamentous growth. Taken together, these data indicate that Clb2p and Clb4p regulate C. albicans morphogenesis by negatively regulating polarized growth.

Present address: MD Biosciences, St. Paul, MN 55108.

Address correspondence to: Judith Berman (judith{at}cbs.umn.edu).

This article has been cited by other articles:

				R. K. Sherwood and R. J. Bennett Microtubule Motor Protein Kar3 Is Required for Normal Mitotic Division and Morphogenesis in Candida albicans Eukaryot. Cell, September 1, 2008; 7(9): 1460 - 1474. [Abstract] [Full Text] [PDF]

				K. R. Finley, K. J. Bouchonville, A. Quick, and J. Berman Dynein-dependent nuclear dynamics affect morphogenesis in Candida albicans by means of the Bub2p spindle checkpoint J. Cell Sci., February 15, 2008; 121(4): 466 - 476. [Abstract] [Full Text] [PDF]

				S. Biswas, P. Van Dijck, and A. Datta Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans Microbiol. Mol. Biol. Rev., June 1, 2007; 71(2): 348 - 376. [Abstract] [Full Text] [PDF]

				A. Dunkler and J. Wendland Candida albicans Rho-Type GTPase-Encoding Genes Required for Polarized Cell Growth and Cell Separation Eukaryot. Cell, May 1, 2007; 6(5): 844 - 854. [Abstract] [Full Text] [PDF]

				A. K. Hungerbuehler, P. Philippsen, and A. S. Gladfelter Limited Functional Redundancy and Oscillation of Cyclins in Multinucleated Ashbya gossypii Fungal Cells Eukaryot. Cell, March 1, 2007; 6(3): 473 - 486. [Abstract] [Full Text] [PDF]

				Q.-M. Shi, Y.-M. Wang, X.-D. Zheng, R. Teck Ho Lee, and Y. Wang Critical Role of DNA Checkpoints in Mediating Genotoxic-Stress-induced Filamentous Growth in Candida albicans Mol. Biol. Cell, March 1, 2007; 18(3): 815 - 826. [Abstract] [Full Text] [PDF]

				A. Wang, S. Lane, Z. Tian, A. Sharon, I. Hazan, and H. Liu Temporal and Spatial Control of HGC1 Expression Results in Hgc1 Localization to the Apical Cells of Hyphae in Candida albicans Eukaryot. Cell, February 1, 2007; 6(2): 253 - 261. [Abstract] [Full Text] [PDF]

				A. Clemente-Blanco, A. Gonzalez-Novo, F. Machin, D. Caballero-Lima, L. Aragon, M. Sanchez, C. R. V. de Aldana, J. Jimenez, and J. Correa-Bordes The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans J. Cell Sci., March 15, 2006; 119(6): 1130 - 1143. [Abstract] [Full Text] [PDF]