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Vol. 16, Issue 8, 3574-3590, August 2005

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Association of an A-Kinase-anchoring Protein Signaling Scaffold with Cadherin Adhesion Molecules in Neurons and Epithelial Cells

Jessica A. Gorski ^*, Lisa L. Gomez ^*, John D. Scott , and Mark L. Dell'Acqua ^* 

^* Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045; Program in Neuroscience, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045; and Howard Hughes Medical Institute, Vollum Institute, Oregon Health Sciences University, Portland, OR 97201

Submitted February 16, 2005; Revised May 17, 2005; Accepted May 19, 2005
Monitoring Editor: Asma Nusrat

A-kinase-anchoring protein (AKAP) 79/150 organizes a scaffold of cAMP-dependent protein kinase (PKA), protein kinase C (PKC), and protein phosphatase 2B/calcineurin that regulates phosphorylation pathways underlying neuronal long-term potentiation and long-term depression (LTD) synaptic plasticity. AKAP79/150 postsynaptic targeting requires three N-terminal basic domains that bind F-actin and acidic phospholipids. Here, we report a novel interaction of these domains with cadherin adhesion molecules that are linked to actin through -catenin (-cat) at neuronal synapses and epithelial adherens junctions. Mapping the AKAP binding site in cadherins identified overlap with -cat binding; however, no competition between AKAP and -cat binding to cadherins was detected in vitro. Accordingly, AKAP79/150 exhibited polarized localization with -cat and cadherins in epithelial cell lateral membranes, and -cat was present in AKAP–cadherin complexes isolated from epithelial cells, cultured neurons, and rat brain synaptic membranes. Inhibition of epithelial cell cadherin adhesion and actin polymerization redistributed intact AKAP–cadherin complexes from lateral membranes to intracellular compartments. In contrast, stimulation of neuronal pathways implicated in LTD that depolymerize postsynaptic F-actin disrupted AKAP–cadherin interactions and resulted in loss of the AKAP, but not cadherins, from synapses. This neuronal regulation of AKAP79/150 targeting to cadherins may be important in functional and structural synaptic modifications underlying plasticity.

Abbreviations used: AKAP, A-kinase-anchoring protein; AMPAR, -amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor; -cat, -catenin; -cat, -catenin; CaN, protein phosphatase 2B/calcineurin; Ecad, E-cadherin; FRET, fluorescence resonance energy transfer; LTD, long-term depression; LTP, long-term potentiation; MAGUK, membrane-associated guanylate kinase; MARCKS, myrostoylated lanine-rich C-kinase substrate; Ncad, N-cadherin; NMDA, N-methyl-D-aspartate; PBcad, PB-cadherin; PDZ, postsynaptic density-95, discs large, ZO-1; PKA, cAMP-dependent protein kinase; PIP₂, phosphatidylinositol-4,5-bisphosphate; PSD, postsynaptic density; SAP97, synapse-associated protein 97.

Address correspondence to: Mark L. Dell'Acqua (mark.dellacqua{at}uchsc.edu).

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