QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 16, Issue 9, 4225-4230, September 2005

This Article Full Text Full Text (PDF) Supplemental Table 1 All Versions of this Article: E05-02-0116v1 16/9/4225    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martin, M. Articles by Saxton, W. M. Search for Related Content PubMed PubMed Citation Articles by Martin, M. Articles by Saxton, W. M.

Abl Tyrosine Kinase and Its Substrate Ena/VASP Have Functional Interactions with Kinesin-1

MaryAnn Martin ^* , Shawn M. Ahern-Djamali  , F. Michael Hoffmann , and William M. Saxton ^*

^* Department of Biology, Indiana University, Bloomington, IN 47405; McArdle Laboratory, University of Wisconsin, Madison, WI 53706

Submitted February 10, 2005; Revised June 13, 2005; Accepted June 15, 2005
Monitoring Editor: John Pringle

Relatively little is known about how microtubule motors are controlled or about how the functions of different cytoskeletal systems are integrated. A yeast two-hybrid screen for proteins that bind to Drosophila Enabled (Ena), an actin polymerization factor that is negatively regulated by Abl tyrosine kinase, identified kinesin heavy chain (Khc), a member of the kinesin-1 subfamily of microtubule motors. Coimmunoprecipitation from Drosophila cytosol confirmed a physical interaction between Khc and Ena. Kinesin-1 motors can carry organelles and other macromolecular cargoes from neuronal cell bodies toward terminals in fast-axonal-transport. Ena distribution in larval axons was not affected by mutations in the Khc gene, suggesting that Ena is not itself a fast transport cargo of Drosophila kinesin-1. Genetic interaction tests showed that in a background sensitized by reduced Khc gene dosage, a reduction in Abl gene dosage caused distal paralysis and axonal swellings. A concomitant reduction in ena dosage rescued those defects. These results suggest that Ena/VASP, when not inhibited by the Abl pathway, can bind Khc and reduce its transport activity in axons.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

These authors contributed equally to this work.

Address correspondence to: William Saxton (bsaxton{at}bio.indiana.edu).

This article has been cited by other articles:

				T.-Y. Lin, C.-H. Huang, H.-H. Kao, G.-G. Liou, S.-R. Yeh, C.-M. Cheng, M.-H. Chen, R.-L. Pan, and J.-L. Juang Abi plays an opposing role to Abl in Drosophila axonogenesis and synaptogenesis Development, September 15, 2009; 136(18): 3099 - 3107. [Abstract] [Full Text] [PDF]

				G. Yogalingam and A. M. Pendergast Abl Kinases Regulate Autophagy by Promoting the Trafficking and Function of Lysosomal Components J. Biol. Chem., December 19, 2008; 283(51): 35941 - 35953. [Abstract] [Full Text] [PDF]

				N. Hirokawa and Y. Noda Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics Physiol Rev, July 1, 2008; 88(3): 1089 - 1118. [Abstract] [Full Text] [PDF]

				V. L. Goss, K. A. Lee, A. Moritz, J. Nardone, E. J. Spek, J. MacNeill, J. Rush, M. J. Comb, and R. D. Polakiewicz A common phosphotyrosine signature for the Bcr-Abl kinase Blood, June 15, 2006; 107(12): 4888 - 4897. [Abstract] [Full Text] [PDF]

				J. G. Gindhart Towards an understanding of kinesin-1 dependent transport pathways through the study of protein-protein interactions Briefings in Functional Genomics, March 1, 2006; 5(1): 74 - 86. [Abstract] [Full Text] [PDF]

				P. J. Hollenbeck and W. M. Saxton The axonal transport of mitochondria J. Cell Sci., December 1, 2005; 118(23): 5411 - 5419. [Abstract] [Full Text] [PDF]