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Vol. 17, Issue 1, 213-226, January 2006

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A Genomewide Screen for Petite-negative Yeast Strains Yields a New Subunit of the i-AAA Protease Complex

Cory D. Dunn ^*, Marina S. Lee  , Forrest A. Spencer , and Robert E. Jensen ^*

^* Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205; Departments of McKusick-Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205

Submitted June 30, 2005; Revised October 19, 2005; Accepted October 21, 2005
Monitoring Editor: Thomas Fox

Unlike many other organisms, the yeast Saccharomyces cerevisiae can tolerate the loss of mitochondrial DNA (mtDNA). Although a few proteins have been identified that are required for yeast cell viability without mtDNA, the mechanism of mtDNA-independent growth is not completely understood. To probe the relationship between the mitochondrial genome and cell viability, we conducted a microarray-based, genomewide screen for mitochondrial DNA-dependent yeast mutants. Among the several genes that we discovered is MGR1, which encodes a novel subunit of the i-AAA protease complex located in the mitochondrial inner membrane. mgr1 mutants retain some i-AAA protease activity, yet mitochondria lacking Mgr1p contain a misassembled i-AAA protease and are defective for turnover of mitochondrial inner membrane proteins. Our results highlight the importance of the i-AAA complex and proteolysis at the inner membrane in cells lacking mitochondrial DNA.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Present address: Genetics and Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.

Address correspondence to: Robert E. Jensen (rjensen{at}jhmi.edu).

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