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Vol. 17, Issue 1, 336-344, January 2006
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* Laboratory for Molecular Biology, University of Wisconsin, Madison, WI 53706;
Department of Genetics, University of Wisconsin, Madison, WI 53706; and
Department of Anatomy, University of Wisconsin, Madison, WI 53706
Submitted September 20, 2005;
Accepted October 20, 2005
Monitoring Editor: Martin Chalfie
The division of one cell into two requires the coordination of multiple components. We describe a gene, car-1, whose product may provide a link between disparate cellular processes. Inhibition of car-1 expression in Caenorhabditis elegans embryos causes late cytokinesis failures: cleavage furrows ingress but subsequently regress and the spindle midzone fails to form, even though midzone components are present. The localized accumulation of membrane that normally develops at the apex of the cleavage furrow during the final phase of cytokinesis does not occur and organization of the endoplasmic reticulum is aberrant, indicative of a disruption in membrane trafficking. The car-1 gene has homologues in a number of species, including proteins that associate with RNA binding proteins. CAR-1 localizes to P-granules (germ-line specific ribonucleoprotein particles) and discrete, developmentally regulated cytoplasmic foci. These foci also contain DCAP-1, a protein involved in decapping mRNAs. Thus, CAR-1, a protein likely to be associated with RNA metabolism, plays an essential role in the late stage of cytokinesis, suggesting a novel link between RNA, membrane trafficking and cytokinesis in the C. elegans embryo.
Abbreviations used: RNAi, dsRNA interference; RNP, ribonucleoprotein; ER, endoplasmic reticulum.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: John G. White (jwhite1{at}wisc.edu).
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