QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 17, Issue 1, 525-538, January 2006

This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: E05-05-0447v1 17/1/525    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mardones, G. A. Articles by Howell, K. E. Search for Related Content PubMed PubMed Citation Articles by Mardones, G. A. Articles by Howell, K. E.

Cis-Golgi Matrix Proteins Move Directly to Endoplasmic Reticulum Exit Sites by Association with Tubules

Gonzalo A. Mardones ^*, Christopher M. Snyder , and Kathryn E. Howell

Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045

Submitted May 23, 2005; Revised September 30, 2005; Accepted October 14, 2005
Monitoring Editor: Benjamin Glick

The role of cis-medial Golgi matrix proteins in retrograde traffic is poorly understood. We have used imaging techniques to understand the relationship between the cis-medial Golgi matrix and transmembrane proteins during retrograde traffic in control and brefeldin A (BFA)-treated cells. All five of the cis-medial matrix proteins tested were associated with retrograde tubules within 2-3 min of initiation of tubule formation. Then, at later time points (3-10 min), transmembrane proteins are apparent in the same tubules. Strikingly, both the matrix proteins and the transmembrane proteins moved directly to endoplasmic reticulum (ER) exit sites labeled with p58 and Sec13, and there seemed to be a specific interaction between the ER exit sites and the tips or branch points of the tubules enriched for the matrix proteins. After the initial interaction, Golgi matrix proteins accumulated rapidly (5-10 min) at ER exit sites, and Golgi transmembrane proteins accumulated at the same sites 2 h later. Our data suggest that Golgi cis-medial matrix proteins participate in Golgi-to-ER traffic and play a novel role in tubule formation and targeting.

Abbreviations used: BFA, brefeldin A; GalT, galactosyltransferase; Man-II, mannosidase II.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

^* Present address: Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892

Present address: Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239.

Address correspondence to: Kathryn E. Howell (kathryn.howell{at}uchsc.edu).

This article has been cited by other articles:

				R. K. Morikawa, J. Aoki, F. Kano, M. Murata, A. Yamamoto, M. Tsujimoto, and H. Arai Intracellular Phospholipase A1{gamma} (iPLA1{gamma}) Is a Novel Factor Involved in Coat Protein Complex I- and Rab6-independent Retrograde Transport between the Endoplasmic Reticulum and the Golgi Complex J. Biol. Chem., September 25, 2009; 284(39): 26620 - 26630. [Abstract] [Full Text] [PDF]

				E. Reynders, F. Foulquier, E. Leao Teles, D. Quelhas, W. Morelle, C. Rabouille, W. Annaert, and G. Matthijs Golgi function and dysfunction in the first COG4-deficient CDG type II patient Hum. Mol. Genet., September 1, 2009; 18(17): 3244 - 3256. [Abstract] [Full Text] [PDF]

				T. Iinuma, T. Aoki, K. Arasaki, H. Hirose, A. Yamamoto, R. Samata, H.-P. Hauri, N. Arimitsu, M. Tagaya, and K. Tani Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains J. Cell Sci., May 15, 2009; 122(10): 1680 - 1690. [Abstract] [Full Text] [PDF]

				M. L. Styers, A. K. O'Connor, R. Grabski, E. Cormet-Boyaka, and E. Sztul Depletion of {beta}-COP reveals a role for COP-I in compartmentalization of secretory compartments and in biosynthetic transport of caveolin-1 Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1485 - C1498. [Abstract] [Full Text] [PDF]

				T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940. [Abstract] [Full Text] [PDF]

				C. Williams, R. Choudhury, E. McKenzie, and M. Lowe Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway J. Cell Sci., November 15, 2007; 120(22): 3941 - 3951. [Abstract] [Full Text] [PDF]

				G. A. Mardones, P. V. Burgos, D. A. Brooks, E. Parkinson-Lawrence, R. Mattera, and J. S. Bonifacino The Trans-Golgi Network Accessory Protein p56 Promotes Long-Range Movement of GGA/Clathrin-containing Transport Carriers and Lysosomal Enzyme Sorting Mol. Biol. Cell, September 1, 2007; 18(9): 3486 - 3501. [Abstract] [Full Text] [PDF]

				S. Jiang, S. W. Rhee, P. A. Gleeson, and B. Storrie Capacity of the Golgi Apparatus for Cargo Transport Prior to Complete Assembly Mol. Biol. Cell, September 1, 2006; 17(9): 4105 - 4117. [Abstract] [Full Text] [PDF]

				R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]