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Vol. 17, Issue 10, 4576-4583, October 2006

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The Neurospora crassa White Collar-1 dependent Blue Light Response Requires Acetylation of Histone H3 Lysine 14 by NGF-1

Benedetto Grimaldi^*,, Pierluca Coiro^*,, Patrizia Filetici, Emanuela Berge^||,¶, Joseph R. Dobosy^||,#, Michael Freitag^||,@, Eric U. Selker^||, and Paola Ballario^*,

*Dipartimento di Genetica e Biologia Molecolare and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale Delle Ricerche, Università di Roma "La Sapienza," 00185 Rome, Italy; and ^||Institute of Molecular Biology, University of Oregon, Eugene, OR 97403

Submitted March 24, 2006; Revised July 31, 2006; Accepted August 4, 2006
Monitoring Editor: William Tansey

Blue light-induced transcription in Neurospora crassa is regulated by the White Collar-1 (WC-1) photoreceptor. We report that residue K14 of histone H3 associated with the light-inducible albino-3 (al-3) promoter becomes transiently acetylated after photoinduction. This acetylation depends on WC-1. The relevance of this chromatin modification was directly evaluated in vivo by construction of a Neurospora strain with a mutated histone H3 gene (hH3^K14Q). This strain phenocopies a wc-1 blind mutant and shows a strong reduction of light-induced transcriptional activation of both al-3 and vivid (vvd), another light-inducible gene. We mutated Neurospora GCN Five (ngf-1), which encodes a homologue of the yeast HAT Gcn5p, to generate a strain impaired in H3 K14 acetylation and found that it was defective in photoinduction. Together, our findings reveal a direct link between histone modification and light signaling in Neurospora and contribute to the developing understanding of the molecular mechanisms operating in light-inducible gene activation.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-03-0232) on August 16, 2006.

Present addresses: University of California, Irvine, CA 92697;

Charitè University of Berlin, D-10098 Berlin, Germany;

^# University of Wisconsin, Madison, WI 53792;

^¶ MitoSciences Inc., Eugene, OR 97403;

^@ Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331.

Address correspondence to: Paola Ballario (paola.ballario{at}uniroma1.it)

Abbreviations used: HAT, histone acetyltransferase; LRR, light-responsive region; WCC, White Collar complex.

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