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Originally published as MBC in Press, 10.1091/mbc.E06-04-0365 on September 13, 2006

Vol. 17, Issue 11, 4866-4875, November 2006

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Mechanically Induced Actin-mediated Rocketing of PhagosomesFormula

Margaret Clarke*, Annette Müller-Taubenberger{dagger},{ddagger}, Kurt I. Anderson§, Ulrike Engel||, and Günther Gerisch{dagger}

*Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73121; {dagger}Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany; §Max-Planck-Institut of Molecular Cell Biology and Genetics, 01037 Dresden, Germany; and ||University of Heidelberg, Nikon Imaging Center, 69120 Heidelberg, Germany

Submitted April 28, 2006; Revised August 15, 2006; Accepted September 1, 2006
Monitoring Editor: David Drubin

Actin polymerization can be induced in Dictyostelium by compressing the cells to bring phagosomes filled with large particles into contact with the plasma membrane. Asymmetric actin assembly results in rocketing movement of the phagosomes. We show that the compression-induced assembly of actin at the cytoplasmic face of the plasma membrane involves the Arp2/3 complex. We also identify two other proteins associated with the mechanically induced actin assembly. The class I myosin MyoB accumulates at the plasma membrane–phagosome interface early during the initiation of the response, and coronin is recruited as the actin filaments are disassembling. The forces generated by rocketing phagosomes are sufficient to push the entire microtubule apparatus forward and to dislocate the nucleus.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-04-0365) on September 13, 2006.

{ddagger} Present address: Institute for Cell Biology (ABI), Ludwig Maximilians University Munich, 80336 München, Germany.

Address correspondence to: Margaret Clarke (clarkem{at}omrf.ouhsc.edu)




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