![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 17, Issue 2, 955-965, February 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Departments of Biochemistry and Molecular Biology and Genetics, University of Georgia, Athens, GA 30602
Submitted September 29, 2005;
Revised November 22, 2005;
Accepted November 28, 2005
Monitoring Editor: Marvin P. Wickens
Telomerase synthesizes telomeres at the ends of human chromosomes during S phase. The results presented here suggest that telomerase activity may be regulated by intranuclear trafficking of the key components of the enzyme in human cells. We examined the subcellular localization of endogenous human telomerase RNA (hTR) and telomerase reverse transcriptase (hTERT) in HeLa cervical carcinoma cells. Throughout most of the cell cycle, we found that the two essential components of telomerase accumulate at intranuclear sites separate from telomeres. However, during S phase, both hTR and hTERT are specifically recruited to subsets of telomeres. The localization of telomerase to telomeres is dynamic, peaking at mid-S phase. We also found complex associations of both hTR and hTERT with nucleoli and Cajal bodies during S phase, implicating both structures in the biogenesis and trafficking of telomerase. Our results mark the first observation of human telomerase at telomeres and provide a mechanism for the cell cycle-dependent regulation of telomere synthesis in human cells.
Address correspondence to: Rebecca M. Terns (rterns{at}bmb.uga.edu) and Michael P. Terns (mterns{at}bmb.uga.edu).
This article has been cited by other articles:
|
|
 |
|
  J. L. Osterhage and K. L. Friedman Chromosome End Maintenance by Telomerase J. Biol. Chem., June 12, 2009; 284(24): 16061 - 16065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hearst, A. S. Gilder, S. S. Negi, M. D. Davis, E. M. George, A. A. Whittom, C. G. Toyota, A. Husedzinovic, O. J. Gruss, and M. D. Hebert Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines J. Cell Sci., June 1, 2009; 122(11): 1872 - 1881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Schober, H. Ferreira, V. Kalck, L. R. Gehlen, and S. M. Gasser Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombination Genes & Dev., April 15, 2009; 23(8): 928 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Venteicher, E. B. Abreu, Z. Meng, K. E. McCann, R. M. Terns, T. D. Veenstra, M. P. Terns, and S. E. Artandi A Human Telomerase Holoenzyme Protein Required for Cajal Body Localization and Telomere Synthesis Science, January 30, 2009; 323(5914): 644 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zhang, Y. X. Bai, H. H. Ma, F. Feng, R. Jin, Z. L. Wang, J. Lin, S. P. Sun, P. Yang, X. X. Wang, et al. Silencing PinX1 Compromises Telomere Length Maintenance As Well As Tumorigenicity in Telomerase-Positive Human Cancer Cells Cancer Res., January 1, 2009; 69(1): 75 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bellon and C. Nicot Central role of PI3K in transcriptional activation of hTERT in HTLV-I-infected cells Blood, October 1, 2008; 112(7): 2946 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Tomlinson, E. B. Abreu, T. Ziegler, H. Ly, C. M. Counter, R. M. Terns, and M. P. Terns Telomerase Reverse Transcriptase Is Required for the Localization of Telomerase RNA to Cajal Bodies and Telomeres in Human Cancer Cells Mol. Biol. Cell, September 1, 2008; 19(9): 3793 - 3800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lin, R. Jin, B. Zhang, H. Chen, Y. X. Bai, P. X. Yang, S. W. Han, Y. H. Xie, P. T. Huang, C. Huang, et al. Nucleolar localization of TERT is unrelated to telomerase function in human cells J. Cell Sci., July 1, 2008; 121(13): 2169 - 2176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xie, A. Mosig, X. Qi, Y. Li, P. F. Stadler, and J. J.-L. Chen Structure and Function of the Smallest Vertebrate Telomerase RNA from Teleost Fish J. Biol. Chem., January 25, 2008; 283(4): 2049 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Mizuno, S. Khurts, T. Seki, Y. Hirota, S. Kaneko, and S. Murakami Human Telomerase Exists in Two Distinct Active Complexes In Vivo J. Biochem., May 1, 2007; 141(5): 641 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hoareau-Aveilla, M. Bonoli, M. Caizergues-Ferrer, and Y. Henry hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase RNA, May 1, 2006; 12(5): 832 - 840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. LI and E.H. BLACKBURN Expression and Suppression of Human Telomerase RNA Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 211 - 215. [Abstract] [PDF]
|
|
|
|
|
|
M. TERNS and R. TERNS Noncoding RNAs of the H/ACA Family Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 395 - 405. [Abstract] [PDF]
|
|
|
|
|
|
T. KISS, E. FAYET, B.E. JADY, P. RICHARD, and M. WEBER Biogenesis and Intranuclear Trafficking of Human Box C/D and H/ACA RNPs Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 407 - 417. [Abstract] [PDF]
|
|
|
|
|
|
C.S. PIKAARD Cell Biology of the Arabidopsis Nuclear siRNA Pathway for RNA-directed Chromatin Modification Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 473 - 480. [Abstract] [PDF]
|
|
|
|
|
|
G.J. HANNON, F.V. RIVAS, E.P. MURCHISON, and J.A. STEITZ The Expanding Universe of Noncoding RNAs Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 551 - 564. [Abstract] [PDF]
|
|
