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Originally published as MBC in Press, 10.1091/mbc.E05-08-0796 on December 21, 2005

Vol. 17, Issue 3, 1184-1193, March 2006

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Balance between Transcription and RNA Degradation Is Vital for Saccharomyces cerevisiae Mitochondria: Reduced Transcription Rescues the Phenotype of Deficient RNA Degradation

Agata T. Rogowska * {dagger}, Olga Puchta *, Anna M. Czarnecka *, Aneta Kaniak {dagger}, Piotr P. Stepien * {dagger}, and Pawel Golik * {dagger}

* Department of Genetics, Warsaw University, 02-106 Warsaw, Poland; {dagger} Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland

Submitted August 24, 2005; Accepted December 6, 2005
Monitoring Editor: Thomas Fox

The Saccharomyces cerevisiae SUV3 gene encodes the helicase component of the mitochondrial degradosome (mtEXO), the principal 3'-to-5' exoribonuclease of yeast mitochondria responsible for RNA turnover and surveillance. Inactivation of SUV3 (suv3{Delta}) causes multiple defects related to overaccumulation of aberrant transcripts and precursors, leading to a disruption of mitochondrial gene expression and loss of respiratory function. We isolated spontaneous suppressors that partially restore mitochondrial function in suv3{Delta} strains devoid of mitochondrial introns and found that they correspond to partial loss-of-function mutations in genes encoding the two subunits of the mitochondrial RNA polymerase (Rpo41p and Mtf1p) that severely reduce the transcription rate in mitochondria. These results show that reducing the transcription rate rescues defects in RNA turnover and demonstrates directly the vital importance of maintaining the balance between RNA synthesis and degradation.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05–08–0796) on December 21, 2005.

Address correspondence to: Pawel Golik (pgolik{at}ibb.waw.pl).




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