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Originally published as MBC in Press, 10.1091/mbc.E05-07-0668 on January 25, 2006

Vol. 17, Issue 4, 1959-1970, April 2006

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The Saccharomyces cerevisiae Spindle Pole Body (SPB) Component Nbp1p Is Required for SPB Membrane Insertion and Interacts with the Integral Membrane Proteins Ndc1p and Mps2pFormula

Yasuhiro Araki * {dagger} {ddagger}, Corine K. Lau {dagger} § ||, Hiromi Maekawa * {ddagger}, Sue L. Jaspersen § ¶, Thomas H. Giddings, Jr. §, Elmar Schiebel * {ddagger}, and Mark Winey §

* The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom; § Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347

Submitted July 22, 2005; Revised January 17, 2006; Accepted January 18, 2006
Monitoring Editor: Trisha Davis

The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05-07-0668) on January 25, 2006.

Abbreviations used: CFP, cyan fluorescent protein; DAPI, 4',6-diamidono-2-phenylindole; GFP, green fluorescent protein; NPC, nuclear pore complex; ORF, open reading frame; SPB, spindle pole body; ts, temperature-sensitive.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

{dagger} These authors contributed equally to this work.

{ddagger} Present address: Zentrum, ZMBH, Universität Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany;

|| Present address: Section of Cell and Developmental Biology, Division of Biological Sciences, 0347, University of California, San Diego, La Jolla, CA 92093-0347;

Present address: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO 64110.

Address correspondence to: Mark Winey (Mark.Winey{at}colorado.edu).




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