Molecular Biology of the Cell click for ASCB 2009 Annual Meeting page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E05-11-1013 on February 22, 2006

Vol. 17, Issue 5, 2125-2137, May 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E05-11-1013v1
17/5/2125    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pedram, A.
Right arrow Articles by Levin, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedram, A.
Right arrow Articles by Levin, E. R.

Functional Estrogen Receptors in the Mitochondria of Breast Cancer Cells

Ali Pedram * {dagger}, Mahnaz Razandi * {dagger}, Douglas C. Wallace {ddagger} §, and Ellis R. Levin * {ddagger} ||

* Division of Endocrinology, Veterans Affairs Medical Center, Long Beach, Long Beach, CA 90822; {dagger} Department of Medicine, University of California, Irvine, Irvine CA 92717; {ddagger} Department of Biochemistry, University of California, Irvine, Irvine CA 92717; || Department of Pharmacology, University of California, Irvine, Irvine CA 92717; and § Department of the Center for Molecular and Mitochondrial Medicine and Genetics, University of California, Irvine, Irvine CA 92717

Submitted November 4, 2005; Revised January 26, 2006; Accepted February 15, 2006
Monitoring Editor: M. Bishr Omary

Steroid hormones have been reported to indirectly impact mitochondrial functions, attributed to nuclear receptor-induced production of proteins that localize in this cytoplasmic organelle. Here we show high-affinity estrogen receptors in the mitochondria of MCF-7 breast cancer cells and endothelial cells, compatible with classical estrogen receptors ER{alpha} and ERbeta. We report that in MCF-7, estrogen inhibits UV radiation-induced cytochrome C release, the decrease of the mitochondrial membrane potential, and apoptotic cell death. UV stimulated the formation of mitochondrial reactive oxygen species (mROS), and mROS were essential to inducing mitochondrial events of cell death. mROS mediated the UV activation of c-jun N-terminal kinase (JNK), and protein kinase C (PKC) {delta}, underlying the subsequent translocation of Bax to the mitochondria where oligomerization was promoted. E2 (estradiol) inhibited all these events, directly acting in mitochondria to inhibit mROS by rapidly up-regulating manganese superoxide dismutase activity. We implicate novel functions of ER in the mitochondria of breast cancer that lead to the survival of the tumor cells.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05–11–1013) on February 22, 2006.

Abbreviations used: ER, estrogen receptor; ERK, extracellular-regulated protein kinase; JNK, c-Jun N-terminal kinase; MnSOD, manganese superoxide dismutase; PKC, protein kinase C; ROS, reactive oxygen species.

Address correspondence to: Ellis R. Levin (ellis.levin{at}med.va.gov).




This article has been cited by other articles:


Home page
 Vet PatholHome page
E. Radaelli, A. Arnold, A. Papanikolaou, R. A. Garcia-Fernandez, S. Mattiello, E. Scanziani, and R. D. Cardiff
Mammary Tumor Phenotypes in Wild-Type Aging Female FVB/N Mice with Pituitary Prolactinomas
Vet. Pathol., July 1, 2009; 46(4): 736 - 745.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Strauss, J. Kallio, N. Desai, P. Pakarinen, T. Miettinen, H. Gylling, M. Albrecht, S. Makela, A. Mayerhofer, and M. Poutanen
Increased Exposure to Estrogens Disturbs Maturation, Steroidogenesis, and Cholesterol Homeostasis via Estrogen Receptor {alpha} in Adult Mouse Leydig Cells
Endocrinology, June 1, 2009; 150(6): 2865 - 2872.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. E. Friedman
Regarding "Prostatic hormonal carcinogenesis is mediated by in situ estrogen production and estrogen receptor alpha signaling"
FASEB J, May 1, 2009; 23(5): 1285 - 1286.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
G. Zhang, X. Liu, A. M. Farkas, A. V. Parwani, K. L. Lathrop, D. Lenzner, S. R. Land, and H. Srinivas
Estrogen Receptor {beta} Functions through Nongenomic Mechanisms in Lung Cancer Cells
Mol. Endocrinol., February 1, 2009; 23(2): 146 - 156.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
A. M. Sotoca Covaleda, H. van den Berg, J. Vervoort, P. van der Saag, A. Strom, J.-A. Gustafsson, I. Rietjens, and A. J. Murk
Influence of Cellular ER{alpha}/ER{beta} Ratio on the ER{alpha}-Agonist Induced Proliferation of Human T47D Breast Cancer Cells
Toxicol. Sci., October 1, 2008; 105(2): 303 - 311.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. M. Shaaban, A. R. Green, S. Karthik, Y. Alizadeh, T. A. Hughes, L. Harkins, I. O. Ellis, J. F. Robertson, E. C. Paish, P. T.K. Saunders, et al.
Nuclear and Cytoplasmic Expression of ER{beta}1, ER{beta}2, and ER{beta}5 Identifies Distinct Prognostic Outcome for Breast Cancer Patients
Clin. Cancer Res., August 15, 2008; 14(16): 5228 - 5235.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Razmara, L. Sunday, C. Stirone, X. B. Wang, D. N. Krause, S. P. Duckles, and V. Procaccio
Mitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor {alpha} in Brain Endothelial Cells
J. Pharmacol. Exp. Ther., June 1, 2008; 325(3): 782 - 790.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. A. Mattingly, M. M. Ivanova, K. A. Riggs, N. S. Wickramasinghe, M. J. Barch, and C. M. Klinge
Estradiol Stimulates Transcription of Nuclear Respiratory Factor-1 and Increases Mitochondrial Biogenesis
Mol. Endocrinol., March 1, 2008; 22(3): 609 - 622.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A. Vasconsuelo, L. Milanesi, and R. Boland
17{beta}-Estradiol abrogates apoptosis in murine skeletal muscle cells through estrogen receptors: role of the phosphatidylinositol 3-kinase/Akt pathway
J. Endocrinol., February 1, 2008; 196(2): 385 - 397.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
E. Murphy and C. Steenbergen
Gender-based differences in mechanisms of protection in myocardial ischemia-reperfusion injury
Cardiovasc Res, August 1, 2007; 75(3): 478 - 486.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
W. H. Watson and J. D. Yager
Arsenic: Extension of its Endocrine Disruption Potential to Interference with Estrogen Receptor-Mediated Signaling
Toxicol. Sci., July 1, 2007; 98(1): 1 - 4.
[Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Schumacher, R. Guennoun, A. Ghoumari, C. Massaad, F. Robert, M. El-Etr, Y. Akwa, K. Rajkowski, and E.-E. Baulieu
Novel Perspectives for Progesterone in Hormone Replacement Therapy, with Special Reference to the Nervous System
Endocr. Rev., June 1, 2007; 28(4): 387 - 439.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. O'Lone, K. Knorr, I. Z. Jaffe, M. E. Schaffer, P. G. V. Martini, R. H. Karas, J. Bienkowska, M. E. Mendelsohn, and U. Hansen
Estrogen Receptors {alpha} and {beta} Mediate Distinct Pathways of Vascular Gene Expression, Including Genes Involved in Mitochondrial Electron Transport and Generation of Reactive Oxygen Species
Mol. Endocrinol., June 1, 2007; 21(6): 1281 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Zheng, A. Kallio, and P. Harkonen
Tamoxifen-Induced Rapid Death of MCF-7 Breast Cancer Cells Is Mediated via Extracellularly Signal-Regulated Kinase Signaling and Can Be Abrogated by Estrogen
Endocrinology, June 1, 2007; 148(6): 2764 - 2777.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.