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Vol. 17, Issue 5, 2158-2165, May 2006

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The Mcp Element Mediates Stable Long-Range Chromosome–Chromosome Interactions in Drosophila

Julio Vazquez ^* , Martin Müller  , Vincenzo Pirrotta , and John W. Sedat ^||

^* Division of Shared Resources, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; Department of Zoology, University of Basel, CH-4056 Basel, Switzerland; Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08544; and ^|| Department of Biophysics and Biochemistry, University of California, San Francisco, San Francisco, CA 94143-2240

Submitted January 17, 2006; Accepted February 10, 2006
Monitoring Editor: Yixian Zheng

Chromosome organization inside the nucleus is not random but rather is determined by a variety of factors, including interactions between chromosomes and nuclear components such as the nuclear envelope or nuclear matrix. Such interactions may be critical for proper nuclear organization, chromosome partitioning during cell division, and gene regulation. An important, but poorly documented subset, includes interactions between specific chromosomal regions. Interactions of this type are thought to be involved in long-range promoter regulation by distant enhancers or locus control regions and may underlie phenomena such as transvection. Here, we used an in vivo microscopy assay based on Lac Repressor/operator recognition to show that Mcp, a polycomb response element from the Drosophila bithorax complex, is able to mediate physical interaction between remote chromosomal regions. These interactions are tissue specific, can take place between multiple Mcp elements, and seem to be stable once established. We speculate that this ability to interact may be part of the mechanism through which Mcp mediates its regulatory function in the bithorax complex.

Abbreviations used: PRE, polycomb response element.

The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

These authors contributed equally to this work.

Address correspondence to: Julio Vazquez (jvazquez{at}fhcrc.org).

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