QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 17, Issue 5, 2424-2438, May 2006

This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: E05-10-0938v1 17/5/2424    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allersma, M. W. Articles by Holz, R. W. Search for Related Content PubMed PubMed Citation Articles by Allersma, M. W. Articles by Holz, R. W.

Motion Matters: Secretory Granule Motion Adjacent to the Plasma Membrane and Exocytosis

Miriam W. Allersma ^* , Mary A. Bittner ^*, Daniel Axelrod , and Ronald W. Holz ^*

^* Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109-0632; Biophysics Research Division, Department of Physics, University of Michigan, Ann Arbor, MI 48109-0632

Submitted October 11, 2005; Revised February 15, 2006; Accepted February 22, 2006
Monitoring Editor: Jennifer Lippincott-Schwartz

Total internal reflection fluorescence microscopy was used to monitor changes in individual granule motions related to the secretory response in chromaffin cells. Because the motions of granules are very small (tens of nanometers), instrumental noise in the quantitation of granule motion was taken into account. ATP and Ca²⁺, both of which prime secretion before fusion, also affect granule motion. Removal of ATP in permeabilized cells causes average granule motion to decrease. Nicotinic stimulation causes a calcium-dependent increase in average granule motion. This effect is more pronounced for granules that undergo exocytosis than for those that do not. Fusion is not preceded by a reduction in mobility. Granules sometimes move 100 nm or more up to and within a tenth of a second before fusion. Thus, the jittering motion of granules adjacent to the plasma membrane is regulated by factors that regulate secretion and may play a role in secretion. Motion continues until shortly before fusion, suggesting that interaction of granule and plasma membrane proteins is transient. Disruption of actin dynamics did not significantly alter granule motion.

Abbreviations used: DMPP, 1,1 dimethyl-4-phenylpiperzinium; GFP, green fluorescent protein; NaGEP, sodium glutamate solution with EGTA and PIPES; PSS, physiological salt solution; R motion, motion in the plane of the coverslip (x,y); TIRFM, total internal reflection fluorescence microscopy.

Address correspondence to: Miriam W. Allersma (allersma{at}umich.edu).

This article has been cited by other articles:

				C. A. Furman, R. Chen, B. Guptaroy, M. Zhang, R. W. Holz, and M. Gnegy Dopamine and Amphetamine Rapidly Increase Dopamine Transporter Trafficking to the Surface: Live-Cell Imaging Using Total Internal Reflection Fluorescence Microscopy J. Neurosci., March 11, 2009; 29(10): 3328 - 3336. [Abstract] [Full Text] [PDF]

				M. Camacho, J. D. Machado, J. Alvarez, and R. Borges Intravesicular Calcium Release Mediates the Motion and Exocytosis of Secretory Organelles: A STUDY WITH ADRENAL CHROMAFFIN CELLS J. Biol. Chem., August 15, 2008; 283(33): 22383 - 22389. [Abstract] [Full Text] [PDF]

				V. E. Degtyar, M. W. Allersma, D. Axelrod, and R. W. Holz Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion PNAS, October 2, 2007; 104(40): 15929 - 15934. [Abstract] [Full Text] [PDF]

				C. Desnos, S. Huet, I. Fanget, C. Chapuis, C. Bottiger, V. Racine, J.-B. Sibarita, J.-P. Henry, and F. Darchen Myosin Va Mediates Docking of Secretory Granules at the Plasma Membrane J. Neurosci., September 26, 2007; 27(39): 10636 - 10645. [Abstract] [Full Text] [PDF]

				M. Wu, T. Baumgart, S. Hammond, D. Holowka, and B. Baird Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays J. Cell Sci., September 1, 2007; 120(17): 3147 - 3154. [Abstract] [Full Text] [PDF]

				A. Gulyas-Kovacs, H. de Wit, I. Milosevic, O. Kochubey, R. Toonen, J. Klingauf, M. Verhage, and J. B. Sorensen Munc18-1: Sequential Interactions with the Fusion Machinery Stimulate Vesicle Docking and Priming J. Neurosci., August 8, 2007; 27(32): 8676 - 8686. [Abstract] [Full Text] [PDF]

				D. Shakiryanova, M. K. Klose, Y. Zhou, T. Gu, D. L. Deitcher, H. L. Atwood, R. S. Hewes, and E. S. Levitan Presynaptic Ryanodine Receptor-Activated Calmodulin Kinase II Increases Vesicle Mobility and Potentiates Neuropeptide Release J. Neurosci., July 18, 2007; 27(29): 7799 - 7806. [Abstract] [Full Text] [PDF]

				D. J. Michael, W. Xiong, X. Geng, P. Drain, and R. H. Chow Human Insulin Vesicle Dynamics During Pulsatile Secretion Diabetes, May 1, 2007; 56(5): 1277 - 1288. [Abstract] [Full Text] [PDF]

				S. Nofal, U. Becherer, D. Hof, U. Matti, and J. Rettig Primed Vesicles Can Be Distinguished from Docked Vesicles by Analyzing Their Mobility J. Neurosci., February 7, 2007; 27(6): 1386 - 1395. [Abstract] [Full Text] [PDF]