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Vol. 17, Issue 6, 2537-2546, June 2006

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Compartmentation of the Nucleolar Processing Proteins in the Granular Component Is a CK2-driven Process

Institut Jacques Monod, Centre National de la Recherche Scientifique, University Paris VI and Paris VII, 75251 Paris Cedex 05, France

Submitted October 5, 2005; Revised February 27, 2006; Accepted March 7, 2006
Monitoring Editor: A. Gregory Matera

To analyze the compartmentation of nucleolar protein complexes, the mechanisms controlling targeting of nucleolar processing proteins onto rRNA transcription sites has been investigated. We studied the reversible disconnection of transcripts and processing proteins using digitonin-permeabilized cells in assays capable of promoting nucleolar reorganization. The assays show that the dynamics of nucleolar reformation is ATP/GTP-dependent, sensitive to temperature, and CK2-driven. We further demonstrate the role of CK2 on the rRNA-processing protein B23. Mutation of the major CK2 site on B23 induces reorganization of nucleolar components that separate from each other. This was confirmed in assays using extracts containing B23 mutated in the CK2-binding sites. We propose that phosphorylation controls the compartmentation of the rRNA-processing proteins and that CK2 is involved in this process.

* These authors contributed equally to this work.

Address correspondence to: D. Hernandez-Verdun ( dhernand{at}ccr.jussieu.fr)

Abbreviations used: AMP-PNP, 5'-adenylylimidodiphosphate; CDK, cyclin-dependent kinase; CK2, casein kinase 2; DFC, dense fibrillar component; DRB, 5,6 dichloro-1--D-ribofuranosylbenzimidazole; FC, fibrillar component; GC, granular component; GFP, green fluorescent protein

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