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Originally published as MBC in Press, 10.1091/mbc.E05-10-0917 on April 12, 2006

Vol. 17, Issue 6, 2757-2769, June 2006

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The rab Exchange Factor Sec2p Reversibly Associates with the ExocystFormula

Martina Medkova, Y. Ellen France, Jeff Coleman, and Peter Novick

Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510

Submitted October 4, 2005; Accepted March 31, 2006
Monitoring Editor: Howard Riezman

Activation of the rab GTPase, Sec4p, by its exchange factor, Sec2p, is needed for polarized transport of secretory vesicles to exocytic sites and for exocytosis. A small region in the C-terminal half of Sec2p regulates its localization. Loss of this region results in temperature-sensitive growth and the depolarized accumulation of secretory vesicles. Here, we show that Sec2p associates with the exocyst, an octameric effector of Sec4p involved in tethering secretory vesicles to the plasma membrane. Specifically, the exocyst subunit Sec15p directly interacts with Sec2p. This interaction normally occurs on secretory vesicles and serves to couple nucleotide exchange on Sec4p to the recruitment of the Sec4p effector. The mislocalization of Sec2p mutants correlates with dramatically enhanced binding to the exocyst complex. We propose that Sec2p is normally released from the exocyst after vesicle tethering so that it can recycle onto a new round of vesicles. The mislocalization of Sec2p mutants results from a failure to be released from Sec15p, blocking this recycling pathway.

This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05-10-0917) on April 12, 2006.

Formula The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

Address correspondence to: Peter J. Novick ( peter.novick{at}yale.edu)

Abbreviations used: GEF, guanine nucleotide exchange factor; GFP, green fluorescent protein; GST, glutathione S-transferase; sec, secretory.




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Y. Sato, S. Fukai, R. Ishitani, and O. Nureki
Crystal structure of the Sec4p{middle dot}Sec2p complex in the nucleotide exchanging intermediate state
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Rabs and their effectors: Achieving specificity in membrane traffic
PNAS, August 8, 2006; 103(32): 11821 - 11827.
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